BRENDA - Enzyme Database
show all sequences of 1.1.3.30

Existence of a novel enzyme, pyrroloquinoline quinone-dependent polyvinyl alcohol dehydrogenase, in a bacterial symbiont, Pseudomonas sp. strain VM15C

Shimao, M.; Ninomiyaaa, K.; Kuno, O.; Kato, N.; Sakazawa, C.; Appl. Environ. Microbiol. 51, 268-275 (1986) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
amount depending on growth rate
Pseudomonas sp.
-
-
periplasm
-
Pseudomonas sp.
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
polyvinyl alcohol + O2
Pseudomonas sp.
-
oxidized polyvinyl alcohol + H2O2
-
Pseudomonas sp.
?
polyvinyl alcohol + O2
Pseudomonas sp. VM15C
-
oxidized polyvinyl alcohol + H2O2
-
Pseudomonas sp. VM15C
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
-
in symbiotic culture with Pseudomonas putida during PVA utilization
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture supernatant
-
Pseudomonas sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.68
-
polyvinyl alcohol
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,5-hexanediol + O2
4.9% of activity with polyvinyl alcohol
287625
Pseudomonas sp.
2,5-hexanedione + H2O2
-
287625
Pseudomonas sp.
?
2-hexanol + O2
101% of activity with polyvinyl alcohol
287625
Pseudomonas sp.
2-hexanone + H2O2
-
287625
Pseudomonas sp.
?
2-hexanol + O2
101% of activity with polyvinyl alcohol
287625
Pseudomonas sp. VM15C
2-hexanone + H2O2
-
287625
Pseudomonas sp. VM15C
?
2-pentanol + O2
58% of activity with polyvinyl alcohol
287625
Pseudomonas sp.
2-pentanone + H2O2
-
287625
Pseudomonas sp.
?
2-pentanol + O2
58% of activity with polyvinyl alcohol
287625
Pseudomonas sp. VM15C
2-pentanone + H2O2
-
287625
Pseudomonas sp. VM15C
?
2-propanol + O2
4.9% of activity with polyvinyl alcohol
287625
Pseudomonas sp.
acetone + H2O2
-
287625
Pseudomonas sp.
?
2-propanol + O2
4.9% of activity with polyvinyl alcohol
287625
Pseudomonas sp. VM15C
acetone + H2O2
-
287625
Pseudomonas sp. VM15C
?
4-heptanol + O2
91% of activity with polyvinyl alcohol
287625
Pseudomonas sp.
4-heptanone + H2O2
-
287625
Pseudomonas sp.
?
4-heptanol + O2
91% of activity with polyvinyl alcohol
287625
Pseudomonas sp. VM15C
4-heptanone + H2O2
-
287625
Pseudomonas sp. VM15C
?
polyvinyl alcohol + O2
-
287625
Pseudomonas sp.
oxidized polyvinyl alcohol + H2O2
-
287625
Pseudomonas sp.
?
polyvinyl alcohol + O2
-
287625
Pseudomonas sp. VM15C
oxidized polyvinyl alcohol + H2O2
-
287625
Pseudomonas sp. VM15C
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no requirement for pyrroloquinoline quinone (PQQ) as does the PQQ-dependent PVA dehydrogenase
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no requirement for pyrroloquinoline quinone (PQQ) as does the PQQ-dependent PVA dehydrogenase
Pseudomonas sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
amount depending on growth rate
Pseudomonas sp.
-
-
periplasm
-
Pseudomonas sp.
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
polyvinyl alcohol + O2
Pseudomonas sp.
-
oxidized polyvinyl alcohol + H2O2
-
Pseudomonas sp.
?
polyvinyl alcohol + O2
Pseudomonas sp. VM15C
-
oxidized polyvinyl alcohol + H2O2
-
Pseudomonas sp. VM15C
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture supernatant
-
Pseudomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.68
-
polyvinyl alcohol
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,5-hexanediol + O2
4.9% of activity with polyvinyl alcohol
287625
Pseudomonas sp.
2,5-hexanedione + H2O2
-
287625
Pseudomonas sp.
?
2-hexanol + O2
101% of activity with polyvinyl alcohol
287625
Pseudomonas sp.
2-hexanone + H2O2
-
287625
Pseudomonas sp.
?
2-hexanol + O2
101% of activity with polyvinyl alcohol
287625
Pseudomonas sp. VM15C
2-hexanone + H2O2
-
287625
Pseudomonas sp. VM15C
?
2-pentanol + O2
58% of activity with polyvinyl alcohol
287625
Pseudomonas sp.
2-pentanone + H2O2
-
287625
Pseudomonas sp.
?
2-pentanol + O2
58% of activity with polyvinyl alcohol
287625
Pseudomonas sp. VM15C
2-pentanone + H2O2
-
287625
Pseudomonas sp. VM15C
?
2-propanol + O2
4.9% of activity with polyvinyl alcohol
287625
Pseudomonas sp.
acetone + H2O2
-
287625
Pseudomonas sp.
?
2-propanol + O2
4.9% of activity with polyvinyl alcohol
287625
Pseudomonas sp. VM15C
acetone + H2O2
-
287625
Pseudomonas sp. VM15C
?
4-heptanol + O2
91% of activity with polyvinyl alcohol
287625
Pseudomonas sp.
4-heptanone + H2O2
-
287625
Pseudomonas sp.
?
4-heptanol + O2
91% of activity with polyvinyl alcohol
287625
Pseudomonas sp. VM15C
4-heptanone + H2O2
-
287625
Pseudomonas sp. VM15C
?
polyvinyl alcohol + O2
-
287625
Pseudomonas sp.
oxidized polyvinyl alcohol + H2O2
-
287625
Pseudomonas sp.
?
polyvinyl alcohol + O2
-
287625
Pseudomonas sp. VM15C
oxidized polyvinyl alcohol + H2O2
-
287625
Pseudomonas sp. VM15C
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas sp.
Other publictions for EC 1.1.3.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740326
Li
-
Purification and enzymatic pro ...
Priestia megaterium
Chin. J. Proc. Engin.
14
1015-1019
2014
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
695840
Kawai
Biochemistry of microbial poly ...
Microbacterium barkeri, Paenibacillus amylolyticus, Priestia megaterium, Brevundimonas vesicularis, Penicillium sp., Geotrichum sp., no activity in Alcaligenes faecalis, Pseudomonas sp., Sphingomonas sp., Streptomyces venezuelae, Sphingopyxis sp., no activity in Alcaligenes faecalis KK314, Brevundimonas vesicularis PD, Brevundimonas vesicularis PH, Priestia megaterium BX1, Streptomyces venezuelae GY1, Geotrichum sp. WF9101, Microbacterium barkeri KCCM10507, Paenibacillus amylolyticus KCCM 10508, Penicillium sp. WSH02-21
Appl. Microbiol. Biotechnol.
84
227-237
2009
3
-
-
-
-
-
-
-
1
-
-
18
-
30
-
-
2
-
-
2
-
-
18
-
10
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
18
-
-
-
2
-
2
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684640
Hu
The pva operon is located on t ...
Sphingopyxis sp.
Appl. Microbiol. Biotechnol.
78
685-693
2008
-
-
1
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
-
1
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684619
Vaclavkova
Novel aspects of symbiotic (po ...
Sphingomonas sp., no activity in Rhodococcus erythropolis
Appl. Microbiol. Biotechnol.
76
911-917
2007
1
-
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684752
Hu
Cell surface structure enhanci ...
Sphingopyxis sp.
Arch. Microbiol.
188
235-241
2007
1
-
-
-
-
-
-
-
1
-
-
1
-
5
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
671537
Yamatsu
Isolation and characterization ...
Sphingopyxis sp., Sphingopyxis sp. PVA3
Appl. Microbiol. Biotechnol.
72
804-811
2006
-
-
-
-
-
-
-
-
-
-
-
2
-
8
-
-
-
-
-
1
-
-
4
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
675840
Hirota-Mamoto
Cloning and expression of the ...
Sphingopyxis sp.
Microbiology
152
1941-1949
2006
1
-
1
-
-
-
-
-
1
2
1
-
-
17
-
-
1
-
-
-
1
1
7
2
3
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
2
1
-
-
-
-
1
-
-
1
1
7
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
287626
Kawagoshi
-
Purification and properties of ...
Brevundimonas vesicularis
World J. Microbiol. Biotechnol.
13
273-277
1997
-
-
-
-
-
-
5
-
-
1
2
1
-
1
-
-
1
-
-
-
1
-
15
1
-
1
1
1
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
1
2
1
-
-
-
1
-
-
1
-
15
1
1
1
1
-
1
1
1
-
-
-
-
-
-
-
287624
Shimao
Pyrroloquinoline quinone-depen ...
no activity in Pseudomonas sp., Pseudomonas sp., no activity in Pseudomonas sp. VM15C
Appl. Environ. Microbiol.
55
275-278
1989
-
-
-
-
-
-
-
-
2
-
-
1
-
13
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287625
Shimao
Existence of a novel enzyme, p ...
Pseudomonas sp.
Appl. Environ. Microbiol.
51
268-275
1986
-
-
-
-
-
-
-
-
2
-
1
2
-
6
-
-
1
-
-
1
1
-
11
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
1
2
-
-
-
1
-
1
1
-
11
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
287623
Shimao
Localization of polyvinyl alco ...
Pseudomonas sp.
Appl. Environ. Microbiol.
49
8-10
1985
-
-
-
-
-
-
-
-
2
-
-
3
-
5
-
-
1
-
-
1
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3
-
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-
-
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2
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3
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1
-
1
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3
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-