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Literature summary for 1.1.3.29 extracted from

  • Horiuchi, T.
    Purification and properties of N-acyl-D-hexosamine oxidase from Pseudomonas sp. 15-1 (1989), Agric. Biol. Chem., 53, 361-368.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
SDS slightly accelerates activity Pseudomonas sp.

Application

Application Comment Organism
analysis enzyme may by useful for measurement of N-acetyl-beta-D-glucosaminidase Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ weak Pseudomonas sp.
Cu2+
-
Pseudomonas sp.
Hg2+
-
Pseudomonas sp.
Mn2+
-
Pseudomonas sp.
Ni2+
-
Pseudomonas sp.
oxalate
-
Pseudomonas sp.
PCMB
-
Pseudomonas sp.
Zn2+
-
Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
N-acetyl-D-galactosamine
-
Pseudomonas sp.
0.24
-
N-acetyl-D-glucosamine
-
Pseudomonas sp.
2.6
-
N-Glycolyl-D-glucosamine
-
Pseudomonas sp.
10
-
D-galactosamine
-
Pseudomonas sp.
18
-
N,N-diacetylchitobiose
-
Pseudomonas sp.
33
-
N-acetyl muramic acid
-
Pseudomonas sp.
40
-
D-glucosamine
-
Pseudomonas sp.
40
-
N-acetyl-D-mannosamine
-
Pseudomonas sp.
59
-
D-mannose
-
Pseudomonas sp.
170
-
D-galactose
-
Pseudomonas sp.
290
-
D-glucose
-
Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE Pseudomonas sp.
36000
-
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE Pseudomonas sp.
44000
-
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE Pseudomonas sp.
63000
-
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE Pseudomonas sp.
170000
-
gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. 15-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
70.4
-
N-acetyl-D-glucosamine Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactosamine + O2 35% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. ?
-
?
D-galactose + O2 0.43% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. D-galactono-1,5-lactone + H2O2
-
?
D-galactose + O2 0.43% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. 15-1 D-galactono-1,5-lactone + H2O2
-
?
D-glucosamine + O2 14% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. ?
-
?
D-glucose + O2 0.3% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. D-gluconolactone + H2O2
-
?
D-mannosamine + O2 0.03% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. ?
-
?
D-mannose + O2 0.38% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. D-mannonolactone + H2O2
-
?
N,N-diacetylchitobiose + O2 31% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. ?
-
?
N-acetyl-beta-D-glucosamine + O2 alpha-anomer is not oxidized Pseudomonas sp. N-acetyl-D-glucosaminolactone + H2O2 in presence of H2O: N-acetyl-D-glucosaminate + H2O2 ?
N-acetyl-beta-D-glucosamine + O2 alpha-anomer is not oxidized Pseudomonas sp. 15-1 N-acetyl-D-glucosaminolactone + H2O2 in presence of H2O: N-acetyl-D-glucosaminate + H2O2 ?
N-acetyl-D-galactosamine + O2 98% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. N-acetyl-D-galactosaminolactone + H2O2
-
?
N-acetyl-D-galactosamine + O2 98% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. 15-1 N-acetyl-D-galactosaminolactone + H2O2
-
?
N-acetyl-D-glucosamine + O2
-
Pseudomonas sp. N-acetyl-D-glucosaminate + H2O2
-
r
N-acetyl-D-mannosamine + O2 4.9% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. N-acetyl-D-mannosaminolactone + H2O2
-
?
N-acetyl-D-mannosamine + O2 4.9% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. 15-1 N-acetyl-D-mannosaminolactone + H2O2
-
?
N-acetylmuramic acid + O2 44% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. ?
-
?
N-glycolyl-D-glucosamine + O2 72% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. N-glycolyl-D-glucosaminolactone + H2O2
-
?
N-glycolyl-D-glucosamine + O2 72% of the activity with N-acetyl-D-glucosamine Pseudomonas sp. 15-1 N-glycolyl-D-glucosaminolactone + H2O2
-
?

Subunits

Subunits Comment Organism
tetramer 1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
10 min, stable Pseudomonas sp.
70
-
100% remaining activity after 10 min Pseudomonas sp.
83
-
50% remaining activity after 10 min Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 11 pH 5.0: about 70% of maximal activity, pH 11: about 50% of maximal activity Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 stable at 45°C for 10 min Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
additional information probably contains unidentified flavin component Pseudomonas sp.