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show all sequences of 1.1.3.28

Properties of a new enzyme, nucleoside oxidase, from Pseudomonas maltophilia LB-86

Isono, Y.; Sudo, T.; Hoshino, M.; Agric. Biol. Chem. 53, 1671-1677 (1989)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
HgCl2
30.8% inhibition at 1 mM
Stenotrophomonas maltophilia
KCN
65.2% inhibition at 1 mM, complete inhibition at 10 mM
Stenotrophomonas maltophilia
N-bromosuccinimide
complete inhibition at 1 mM
Stenotrophomonas maltophilia
NaN3
18.5% inhibition at 1 mM
Stenotrophomonas maltophilia
Pb(CH3COO)2
18.7% inhibition at 1 mM
Stenotrophomonas maltophilia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.044
-
Inosine
-
Stenotrophomonas maltophilia
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
2 g non-heme iron per mol of enzyme, contains also a heme component
Stenotrophomonas maltophilia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
18000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
33000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
76000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
130000
-
gel filtration
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
inosine + O2
Stenotrophomonas maltophilia
oxidation of various nucleosides, but no oxidation of nucleotides, bases and ribose
9-riburonosylhypoxanthine + H2O
-
Stenotrophomonas maltophilia
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Stenotrophomonas maltophilia
-
-
-
Stenotrophomonas maltophilia LB-86
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Stenotrophomonas maltophilia
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Stenotrophomonas maltophilia
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-deoxyadenosine + O2
81.4% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-(2-deoxy-beta-D-erythro-pentofuranuronosyl)-9H-purin-6-amine + H2O
-
287612
Stenotrophomonas maltophilia
?
adenosine + O2
97.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosyladenine + H2O
-
287612
Stenotrophomonas maltophilia
?
adenosine + O2
97.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
9-riburonosyladenine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
cytidine + O2
77.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
1-riburonosylcytosine + H2O
-
287612
Stenotrophomonas maltophilia
?
cytidine + O2
77.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
1-riburonosylcytosine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
deoxycytidine + O2
77.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
1-riburonosylcytosine + H2O
-
287612
Stenotrophomonas maltophilia
?
deoxyguanosine + O2
92.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosyldeoxyguanine + H2O
-
287612
Stenotrophomonas maltophilia
?
deoxyinosine + O2
80.7% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosyldeoxyhypoxanthine + H2O
-
287612
Stenotrophomonas maltophilia
?
guanosine + O2
121% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosylguanine + H2O
-
287612
Stenotrophomonas maltophilia
?
guanosine + O2
121% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
9-riburonosylguanine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
inosine + O2
oxidation of various nucleosides, but no oxidation of nucleotides, bases and ribose
287612
Stenotrophomonas maltophilia
9-riburonosylhypoxanthine + H2O
-
287612
Stenotrophomonas maltophilia
?
thymidine + O2
55.8% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
1-riburonosylthymine + H2O
-
287612
Stenotrophomonas maltophilia
?
uridine + O2
-
287612
Stenotrophomonas maltophilia
1-riburonosyluracil + H2O
-
287612
Stenotrophomonas maltophilia
?
uridine + O2
-
287612
Stenotrophomonas maltophilia LB-86
1-riburonosyluracil + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
xanthosine + O2
best substrate tested, 125% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosylxanthine + H2O
-
287612
Stenotrophomonas maltophilia
?
xanthosine + O2
best substrate tested, 125% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
9-riburonosylxanthine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
Subunits
Subunits
Commentary
Organism
heterotetramer
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
stable below, complete loss of activity at 75°C
Stenotrophomonas maltophilia
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
6
-
Stenotrophomonas maltophilia
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
6
stable for 1 h at 37°C
Stenotrophomonas maltophilia
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
1 FAD covalently bound to the alpha subunit
Stenotrophomonas maltophilia
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
1 FAD covalently bound to the alpha subunit
Stenotrophomonas maltophilia
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
HgCl2
30.8% inhibition at 1 mM
Stenotrophomonas maltophilia
KCN
65.2% inhibition at 1 mM, complete inhibition at 10 mM
Stenotrophomonas maltophilia
N-bromosuccinimide
complete inhibition at 1 mM
Stenotrophomonas maltophilia
NaN3
18.5% inhibition at 1 mM
Stenotrophomonas maltophilia
Pb(CH3COO)2
18.7% inhibition at 1 mM
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.044
-
Inosine
-
Stenotrophomonas maltophilia
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
2 g non-heme iron per mol of enzyme, contains also a heme component
Stenotrophomonas maltophilia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
18000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
33000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
76000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
130000
-
gel filtration
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
inosine + O2
Stenotrophomonas maltophilia
oxidation of various nucleosides, but no oxidation of nucleotides, bases and ribose
9-riburonosylhypoxanthine + H2O
-
Stenotrophomonas maltophilia
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Stenotrophomonas maltophilia
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Stenotrophomonas maltophilia
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-deoxyadenosine + O2
81.4% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-(2-deoxy-beta-D-erythro-pentofuranuronosyl)-9H-purin-6-amine + H2O
-
287612
Stenotrophomonas maltophilia
?
adenosine + O2
97.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosyladenine + H2O
-
287612
Stenotrophomonas maltophilia
?
adenosine + O2
97.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
9-riburonosyladenine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
cytidine + O2
77.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
1-riburonosylcytosine + H2O
-
287612
Stenotrophomonas maltophilia
?
cytidine + O2
77.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
1-riburonosylcytosine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
deoxycytidine + O2
77.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
1-riburonosylcytosine + H2O
-
287612
Stenotrophomonas maltophilia
?
deoxyguanosine + O2
92.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosyldeoxyguanine + H2O
-
287612
Stenotrophomonas maltophilia
?
deoxyinosine + O2
80.7% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosyldeoxyhypoxanthine + H2O
-
287612
Stenotrophomonas maltophilia
?
guanosine + O2
121% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosylguanine + H2O
-
287612
Stenotrophomonas maltophilia
?
guanosine + O2
121% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
9-riburonosylguanine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
inosine + O2
oxidation of various nucleosides, but no oxidation of nucleotides, bases and ribose
287612
Stenotrophomonas maltophilia
9-riburonosylhypoxanthine + H2O
-
287612
Stenotrophomonas maltophilia
?
thymidine + O2
55.8% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
1-riburonosylthymine + H2O
-
287612
Stenotrophomonas maltophilia
?
uridine + O2
-
287612
Stenotrophomonas maltophilia
1-riburonosyluracil + H2O
-
287612
Stenotrophomonas maltophilia
?
uridine + O2
-
287612
Stenotrophomonas maltophilia LB-86
1-riburonosyluracil + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
xanthosine + O2
best substrate tested, 125% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosylxanthine + H2O
-
287612
Stenotrophomonas maltophilia
?
xanthosine + O2
best substrate tested, 125% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
9-riburonosylxanthine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterotetramer
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
stable below, complete loss of activity at 75°C
Stenotrophomonas maltophilia
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
6
-
Stenotrophomonas maltophilia
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
6
stable for 1 h at 37°C
Stenotrophomonas maltophilia
Other publictions for EC 1.1.3.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287611
Hoshino
-
Production of a new enzyme, nu ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia LB-86
Agric. Biol. Chem.
53
399-403
1989
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
287612
Isono
-
Properties of a new enzyme, nu ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia LB-86
Agric. Biol. Chem.
53
1671-1677
1989
-
-
-
-
-
-
5
1
-
1
5
1
-
5
-
-
1
-
-
1
-
-
16
1
-
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
5
-
1
-
1
5
1
-
-
-
1
-
1
-
-
16
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
287613
Isono
-
Purification and reaction of a ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia LB-86
Agric. Biol. Chem.
53
1663-1669
1989
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
1
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287614
Isono
-
Laccase-like activity of nucle ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia LB-86
Agric. Biol. Chem.
53
2197-2203
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-