Literature summary for 1.1.3.2 extracted from

Dagar, K.; Pundir, C.
An improved amperometric L-lactate biosensor based on covalent immobilization of microbial lactate oxidase onto carboxylated multiwalled carbon nanotubes/copper nanoparticles/polyaniline modified pencil graphite electrode (2017), Enzyme Microb. Technol., 96, 177-186 .

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Application

Application	Comment	Organism
diagnostics	an improved amperometric L-lactate biosensor was constructed based on covalent immobilization of lactate oxidase from Pediococcus species onto carboxylated multiwalled carbon nanotubes (cMWCNT)/copper nanoparticles (CuNPs)/polyaniline (PANI) hybrid film electrodeposited on the surface of a pencil graphite electrode. The biosensor shows maximum response within 5 s at pH 8.0 in 0.05 M sodium phosphate buffer and 37°C, when operated at 20 mV/s. The biosensor has a detection limit of 0.00025 mM with a wide working range between 0.001-2.5 mM. The biosensor is employed for measurement of L-lactic acid level in plasma of apparently healthy and diseased persons. Analytical recovery of added lactic acid in plasma is 95.5%. The working enzyme electrode is used 180 times over a period of 140 days, when stored at 4°C	Pediococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-lactate + O2	Pediococcus sp.	-	pyruvate + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Pediococcus sp.	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-lactate + O2	-	Pediococcus sp.	pyruvate + H2O2	-	?

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Release 2023.1 (January 2023)