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Literature summary for 1.1.3.2 extracted from

  • Hiraka, K.; Kojima, K.; Tsugawa, W.; Asano, R.; Ikebukuro, K.; Sode, K.
    Rational engineering of Aerococcus viridansl-lactate oxidase for the mediator modification to achieve quasi-direct electron transfer type lactate sensor (2020), Biosens. Bioelectron., 151, 111974 .
    View publication on PubMed
Search for more literature for 1.1.3.2

Application

Application Comment Organism
diagnostics L-lactate oxidase based lactate sensors are widely used for clinical diagnostics, sports medicine, and food quality control. Rational engineering of Aerococcus viridans L-lactate oxidase for the mediator modification to achieve quasi-direct electron transfer type lactate sensor Aerococcus viridans
food industry L-lactate oxidase based lactate sensors are widely used for clinical diagnostics, sports medicine, and food quality control. Rational engineering of Aerococcus viridans L-lactate oxidase for the mediator modification to achieve quasi-direct electron transfer type lactate sensor Aerococcus viridans
medicine L-lactate oxidase based lactate sensors are widely used for clinical diagnostics, sports medicine, and food quality control. Rational engineering of Aerococcus viridans L-lactate oxidase for the mediator modification to achieve quasi-direct electron transfer type lactate sensor Aerococcus viridans

Protein Variants

Protein Variants Comment Organism
A96L the mutant enzyme is more stable than the wild type enzyme and the N212K single mutant Aerococcus viridans
A96L/N212K the double mutant shows a drastic decrease compared with that of the wild type (0.16% using 20 mM L-lactate) and A96L mutant (1.9% using 20 mM L-lactate). The mutant enzyme is more stable than the wild type enzyme and the N212K single mutant. After modification by phenazine ethosulfate, the Ala96Leu/Asn212Lys double mutant shows the highest oxidation peak in the presence of L-lactate, whereas the electrodes with the phenazine ethosulfate-modified wild type or Ala96Leu mutant do not Aerococcus viridans
N212K mutant enzyme shows decreased oxidase activity Aerococcus viridans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
 (S)-lactate pH 7.0, 25°C, wild-type enzyme Aerococcus viridans

Organism

Organism UniProt Comment Textmining
Aerococcus viridans D4YFM2
-
-
Aerococcus viridans ATCC 11563 D4YFM2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + O2
-
 Aerococcus viridans pyruvate + H2O2
-
 ?
(S)-lactate + O2
-
 Aerococcus viridans ATCC 11563 pyruvate + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
L-lactate oxidase
-
 Aerococcus viridans