Protein Variants | Comment | Organism |
---|---|---|
F212V | change in the active site to that of Arabidopsis thaliana glycolate oxidase 2, 25fold decrease in the L-lactate oxidase/glycolate oxidase activity ratio | Nostoc sp. PCC 7120 = FACHB-418 |
L112W | change in the active site to that of Arabidopsis thaliana glycolate oxidase 2, 2fold decrease in the L-lactate oxidase/glycolate oxidase activity ratio | Nostoc sp. PCC 7120 = FACHB-418 |
M82T | change in the active site to that of Arabidopsis thaliana glycolate oxidase 2, 10fold decrease in the L-lactate oxidase/glycolate oxidase activity ratio | Nostoc sp. PCC 7120 = FACHB-418 |
M82T/L112W/F212V | change in the active site to that of Arabidopsis thaliana glycolate oxidase 2, reverse the L-lactate oxidase/glycolate oxidase activity ratio | Nostoc sp. PCC 7120 = FACHB-418 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
L-lactate | pH 8.0, 30°C | Nostoc sp. PCC 7120 = FACHB-418 | |
0.081 | - |
L-lactate | pH 8.0, 30°C | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | F8WQN2 | cf. EC 1.1.3.1, enzyme shows high L-lactate oxidase and low glycolate oxidase activities | - |
Nostoc sp. PCC 7120 = FACHB-418 | Q8Z0C8 | cf. EC 1.1.3.1, enzyme shows high L-lactate oxidase and low glycolate oxidase activities | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerate + O2 | 5.9% of the activity with L-lactate | Nostoc sp. PCC 7120 = FACHB-418 | hydroxypyruvate + H2O2 | - |
? | |
glyoxylate + O2 | 15.3% of the activity with L-lactate | Nostoc sp. PCC 7120 = FACHB-418 | oxalate + H2O2 | - |
? | |
L-lactate + O2 | - |
Nostoc sp. PCC 7120 = FACHB-418 | pyruvate + H2O2 | - |
? | |
L-lactate + O2 | - |
Chlamydomonas reinhardtii | pyruvate + H2O2 | - |
? | |
additional information | no substrate: D-lactate. Poor substrate: glycolate | Nostoc sp. PCC 7120 = FACHB-418 | ? | - |
? | |
additional information | no substrate: D-lactate. Poor substrate: glycolate | Chlamydomonas reinhardtii | ? | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | overexpression decreases the accumulation of toxic glycolate in a cyanobacterial photorespiratory mutant and restores its ability to grow in air. A lactate oxidase-deficient Nostoc sp. mutant grows normally in nitrate-containing medium but dies under N2-fixing conditions. The enzyme may primarily serve as an O2-scavenging enzyme to protect nitrogenase in extant N2-fixing cyanobacteria | Nostoc sp. PCC 7120 = FACHB-418 |