Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.2 extracted from

  • Yorita, K.; Aki, K.; Ohkuma-Soyejima, T.; Kokubo, T.; Misaki, H.; Massey, V.
    Conversion of L-lactate oxidase to a long chain alpha-hydroxyacid oxidase by site-directed mutagenesis of alanine 95 to glycine (1996), J. Biol. Chem., 271, 28300-28305 .
    View publication on PubMed
Search for more literature for 1.1.3.2

Protein Variants

Protein Variants Comment Organism
A95G activity with L-lactate similar to wild-type, increased activity with longer chain L-alpha-hydroxyacids such as alpha-hydroxy-n-butyric acid, alpha-hydroxy-n-valeric acid, and also with L-mandelic acid. Reduction of the enzyme bound flavin by substrates is the rate-limiting step in A95G Aerococcus viridans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 (S)-mandelic acid wild-type, pH 7.0, 25°C Aerococcus viridans
0.87
-
 L-lactate wild-type, pH 7.0, 25°C Aerococcus viridans
5
-
 glycerate wild-type, pH 7.0, 25°C Aerococcus viridans
5.5
-
 2-hydroxypentanoate wild-type, pH 7.0, 25°C Aerococcus viridans
6.75
-
 L-lactate mutant A95G, pH 7.0, 25°C Aerococcus viridans
10
-
 2-hydroxypentanoate mutant A95G, pH 7.0, 25°C Aerococcus viridans
18
-
 2-hydroxybutanoate wild-type, pH 7.0, 25°C Aerococcus viridans
20
-
 (S)-mandelic acid mutant A95G, pH 7.0, 25°C Aerococcus viridans
27
-
 2-hydroxybutanoate mutant A95G, pH 7.0, 25°C Aerococcus viridans
53
-
 glycerate mutant A95G, pH 7.0, 25°C Aerococcus viridans

Organism

Organism UniProt Comment Textmining
Aerococcus viridans Q44467
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-mandelic acid + O2
-
 Aerococcus viridans phenylpyruvate + H2O2
-
 ?
2-hydroxybutanoate + O2
-
 Aerococcus viridans 2-oxobutanoate + H2O2
-
 ?
2-hydroxypentanoate + O2
-
 Aerococcus viridans 2-oxopentanoate + H2O2
-
 ?
glycerate + O2
-
 Aerococcus viridans hydroxypyruvate + H2O2
-
 ?
L-lactate + O2
-
 Aerococcus viridans pyruvate + H2O2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0033
-
 (S)-mandelic acid wild-type, pH 7.0, 25°C Aerococcus viridans
0.87
-
 glycerate wild-type, pH 7.0, 25°C Aerococcus viridans
1.25
-
 2-hydroxypentanoate wild-type, pH 7.0, 25°C Aerococcus viridans
11
-
 2-hydroxybutanoate wild-type, pH 7.0, 25°C Aerococcus viridans
15.8
-
 (S)-mandelic acid mutant A95G, pH 7.0, 25°C Aerococcus viridans
31.3
-
 glycerate mutant A95G, pH 7.0, 25°C Aerococcus viridans
51.7
-
 2-hydroxypentanoate mutant A95G, pH 7.0, 25°C Aerococcus viridans
250
-
 L-lactate mutant A95G, pH 7.0, 25°C Aerococcus viridans
283
-
 L-lactate wild-type, pH 7.0, 25°C Aerococcus viridans
510
-
 2-hydroxybutanoate mutant A95G, pH 7.0, 25°C Aerococcus viridans