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Literature summary for 1.1.3.17 extracted from

  • Finnegan, S.; Yuan, H.; Wang, Y.F.; Orville, A.M.; Weber, I.T.; Gadda, G.
    Structural and kinetic studies on the Ser101Ala variant of choline oxidase: catalysis by compromise (2010), Arch. Biochem. Biophys., 501, 207-213.
    View publication on PubMed
Search for more literature for 1.1.3.17

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzyme S101A is expressed in Escherichia coli Rosetta(DE3)pLysS cells Arthrobacter globiformis

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme S101A, hanging drop vapor diffusion method, using 80 mM sodium cacodylate, 20% (v/v) PEG6000, 20% (v/v) glycerol, 150 mM Mg-acetate at pH 6.0 Arthrobacter globiformis

Protein Variants

Protein Variants Comment Organism
S101A mutant enzyme with increased efficiencies in the oxidative half-reactions and decreased efficiencies in the reductive half-reactions accompanied by a significant decrease in the overall rate of turnover with choline. Ser101 is important, but not essential, for catalysis Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 choline wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis
0.26
-
 choline mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis Q7X2H8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choline + O2
-
 Arthrobacter globiformis betaine aldehyde + H2O2
-
 r

Synonyms

Synonyms Comment Organism
choline-oxygen 1-oxidoreductase
-
 Arthrobacter globiformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.7
-
 choline mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis
43
-
 Betaine aldehyde mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis
60
-
 choline wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis
133
-
 Betaine aldehyde wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Arthrobacter globiformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
25.6
-
 choline mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis
237
-
 choline wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C Arthrobacter globiformis