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Literature summary for 1.1.3.17 extracted from

  • Lopez, M.S.; Perez, J.P.; Lopez-Cabarcos, E.; Lopez-Ruiz, B.
    Amperometric biosensors based on choline oxidase entrapped in polyacrylamide microgels (2007), Electroanalysis, 19, 370-378.
No PubMed abstract available
Search for more literature for 1.1.3.17

Application

Application Comment Organism
biotechnology development and applications of biosensors Alcaligenes sp.

Inhibitors

Inhibitors Comment Organism Structure
Nicotine ability of nicotine to inhibit catalytic activity of choline oxidase used for biosensorial detection of Alcaligenes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information similar level of catalytic activity than free enzyme in solution Alcaligenes sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
choline + O2 Alcaligenes sp.
-
 betaine aldehyde + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes sp. P16101
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Alcaligenes sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 biosensor using choline oxidase entrapped within polyacrylamide microparticles, microgel analysis by Scanning electron microscopy, Galai-Cis particle analyzer, X-ray diffraction and differential scanning calorimetry, influence of the polymer cross-linking content, potential, pH and temperature the biosensor response investigated, design of a bienzyme biosensor by including acetylcholinesterase Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choline + O2
-
 Alcaligenes sp. betaine aldehyde + H2O2
-
 ?
choline + O2 choline detection by mono- and bienzyme biosensors Alcaligenes sp. betaine aldehyde + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
ChOx protein
-
 Alcaligenes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 oxygen saturation, 8.0 mM of choline, 0.1 M Tris-phosphate buffer, pH 9 Alcaligenes sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
18 30 similar to that of the free enzyme ranging between 25°C and 35°C Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 optimum for choline detection, biosensor response examined in 0.1 M phosphate/acetate/TRIS buffer from pH 4.0 to 10.0 at 25°C Alcaligenes sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 biosensor retains 60% of its maximum response Alcaligenes sp.