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Literature summary for 1.1.3.17 extracted from
- Evaluation of the stress-inducible production of choline oxidase in transgenic rice as a strategy for producing the stress-protectant glycine betaine (2006), J. Exp. Bot., 57, 1129-1135.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of the enzyme, fused to a chloroplast-transit peptide from Pisum sativum, in transgenic Oryza sativa plants using the Agrobacterium tumefaciens transfection system | Arthrobacter pascens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | evaluation of the stress-inducible production of Arthrobacter pascens choline oxidase in transgenic rice as a strategy for producing the stress-protectant glycine betaine, stress induction by 150 mM NaCl, biomass production in wild-type and transgenic plants | Arthrobacter pascens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline + O2 | Arthrobacter pascens | - |
betaine aldehyde + H2O2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter pascens | - |
gene COX | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme partially from stressed and non-stressed transgenic rice plant leaves by ammonium sulfate fractionation and desalting gel filtration | Arthrobacter pascens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| choline + O2 = betaine aldehyde + H2O2 | the enzyme catalyzes the oxidation of choline to glycine betaine via the intermediate betaine aldehyde | Arthrobacter pascens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity measurement in a coupled assay with NAD+-dependent betaine aldehyde dehydrogenase, BADH | Arthrobacter pascens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline + O2 | - |
Arthrobacter pascens | betaine aldehyde + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| COX | - |
Arthrobacter pascens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Arthrobacter pascens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Arthrobacter pascens |
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Release 2023.1 (January 2023)
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