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Literature summary for 1.1.3.17 extracted from

  • Yamada, H.; Mori, N.; Tani, Y.
    Properties of choline oxidase of cylindrocarpon didymum M-1 (1979), Agric. Biol. Chem., 43, 2173-2177.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Cylindrocarpon didymum
AgNO3
-
Cylindrocarpon didymum
Cu2+
-
Cylindrocarpon didymum
Hg2+
-
Cylindrocarpon didymum
HgCl2
-
Cylindrocarpon didymum
p-chloromercuribenzoate
-
Cylindrocarpon didymum
Zn2+
-
Cylindrocarpon didymum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
choline
-
Cylindrocarpon didymum
5.8
-
Betaine aldehyde
-
Cylindrocarpon didymum
14
-
N,N-Dimethylaminoethanol
-
Cylindrocarpon didymum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
2 * 64000, SDS-PAGE Cylindrocarpon didymum
120000
-
gel filtration Cylindrocarpon didymum
145000
-
sedimentation velocity method of Svedberg and Pederson Cylindrocarpon didymum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + O2 + H2O Arthrobacter globiformis
-
betaine + H2O2
-
?
betaine aldehyde + O2 + H2O Cylindrocarpon didymum
-
betaine + H2O2
-
?
betaine aldehyde + O2 + H2O Cylindrocarpon didymum M-1
-
betaine + H2O2
-
?
choline + O2 Arthrobacter globiformis
-
betaine aldehyde + H2O2
-
?
choline + O2 Cylindrocarpon didymum
-
betaine aldehyde + H2O2
-
?
choline + O2 Cylindrocarpon didymum M-1
-
betaine aldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
-
-
Cylindrocarpon didymum
-
M-1
-
Cylindrocarpon didymum M-1
-
M-1
-

Purification (Commentary)

Purification (Comment) Organism
-
Cylindrocarpon didymum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + O2 + H2O
-
Arthrobacter globiformis betaine + H2O2
-
?
betaine aldehyde + O2 + H2O
-
Cylindrocarpon didymum betaine + H2O2
-
?
betaine aldehyde + O2 + H2O
-
Cylindrocarpon didymum M-1 betaine + H2O2
-
?
choline + O2
-
Arthrobacter globiformis betaine aldehyde + H2O2
-
?
choline + O2
-
Cylindrocarpon didymum betaine aldehyde + H2O2
-
?
choline + O2
-
Cylindrocarpon didymum M-1 betaine aldehyde + H2O2
-
?
monoethanolamine + O2
-
Cylindrocarpon didymum aminoacetaldehyde + H2O2
-
?
monoethanolamine + O2
-
Cylindrocarpon didymum M-1 aminoacetaldehyde + H2O2
-
?
N,N-dimethylaminoethanol + O2
-
Arthrobacter globiformis (dimethylamino)acetaldehyde + H2O2
-
?
N,N-dimethylaminoethanol + O2
-
Cylindrocarpon didymum (dimethylamino)acetaldehyde + H2O2
-
?
N,N-dimethylaminoethanol + O2
-
Cylindrocarpon didymum M-1 (dimethylamino)acetaldehyde + H2O2
-
?
triethanolamine + O2
-
Arthrobacter globiformis ?
-
?
triethanolamine + O2
-
Cylindrocarpon didymum ?
-
?
triethanolamine + O2
-
Cylindrocarpon didymum M-1 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 64000, SDS-PAGE Cylindrocarpon didymum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Cylindrocarpon didymum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 45 incubated for 10 min at pH 8.5 and 40°C 20% initial activity is lost, 96% at 45°C, only 10% initial activity is lost at 45°C in presence of 10% glycerol Cylindrocarpon didymum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Cylindrocarpon didymum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 50% initial activity is lost at 40°C at pH 6.0 and 9.5 Cylindrocarpon didymum