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Literature summary for 1.1.3.16 extracted from
- Ecdysone oxidase (1985), Methods Enzymol., 111, 419-429.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| stable to repeated freezing and thawing | Calliphora vicina |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methanol | 31% inhibition at 0.5% | Calliphora vicina |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
oxidized 2,6-dichloroindophenol | - |
Calliphora vicina | |
| 0.031 | - |
20-hydroxyecdysone | - |
Calliphora vicina | |
| 0.041 | - |
ecdysone | optical assay | Calliphora vicina | |
| 0.098 | - |
ecdysone | radio assay | Calliphora vicina | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Drosophila melanogaster | 5737 | - |
| cytoplasm | - |
Calliphora vicina | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 240000 | - |
gel filtration | Calliphora vicina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 20-hydroxyecdysone + O2 | Calliphora vicina | probably part of the catabolic system that inactivates the molting hormones | 3-dehydro-20-hydroxyecdysone + H2O2 | - |
? | |
| ecdysone + O2 | Calliphora vicina | probably part of the catabolic system that inactivates the molting hormones | 3-dehydroecdysone + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeshna cyanea | - |
- |
- |
| Calliphora vicina | - |
blue blowfly | - |
| Choristoneura fumiferana | - |
- |
- |
| Drosophila hydei | - |
- |
- |
| Drosophila melanogaster | - |
- |
- |
| Gryllus bimaculatus | - |
- |
- |
| Locusta migratoria | - |
- |
- |
| Tenebrio molitor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Calliphora vicina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pupa | - |
Drosophila melanogaster | - |
| pupa | - |
Calliphora vicina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.2 | - |
- |
Calliphora vicina |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C, more than 3 months | Calliphora vicina |
| 0°C, several days | Calliphora vicina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-20-hydroxyecdysone + O2 | - |
Calliphora vicina | 3-dehydro-2-deoxy-20-hydroxyecdysone + H2O2 | - |
? | |
| 2-deoxyecdysone + O2 | - |
Calliphora vicina | 3-dehydro-2-deoxyecdysone + H2O2 | - |
? | |
| 20-hydroxyecdysone + O2 | probably part of the catabolic system that inactivates the molting hormones | Calliphora vicina | 3-dehydro-20-hydroxyecdysone + H2O2 | - |
? | |
| 5,20-dihydroxyecdysone + O2 | - |
Calliphora vicina | 3-dehydro-5,20-dihydroxyecdysone + H2O2 | - |
? | |
| cyasterone + O2 | - |
Calliphora vicina | 3-dehydrocyasterone + H2O2 | - |
? | |
| ecdysone + O2 | artificial electron acceptors in vitro in ascending order: ferricyanide, methylene blue, 2,6-dichloroindophenol, 5-methazine methyl sulfate | Calliphora vicina | 3-dehydroecdysone + H2O2 | i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione | ir | |
| ecdysone + O2 | 2,6-dichloroindophenol can act as acceptor | Calliphora vicina | 3-dehydroecdysone + H2O2 | i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione | ir | |
| ecdysone + O2 | i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one | Drosophila melanogaster | 3-dehydroecdysone + H2O2 | i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione | ir | |
| ecdysone + O2 | i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one | Tenebrio molitor | 3-dehydroecdysone + H2O2 | i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione | ir | |
| ecdysone + O2 | i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one | Calliphora vicina | 3-dehydroecdysone + H2O2 | i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione | ir | |
| ecdysone + O2 | i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one | Locusta migratoria | 3-dehydroecdysone + H2O2 | i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione | ir | |
| ecdysone + O2 | i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one | Aeshna cyanea | 3-dehydroecdysone + H2O2 | i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione | ir | |
| ecdysone + O2 | i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one | Choristoneura fumiferana | 3-dehydroecdysone + H2O2 | i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione | ir | |
| ecdysone + O2 | i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one | Drosophila hydei | 3-dehydroecdysone + H2O2 | i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione | ir | |
| ecdysone + O2 | i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one | Gryllus bimaculatus | 3-dehydroecdysone + H2O2 | i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione | ir | |
| ecdysone + O2 | probably part of the catabolic system that inactivates the molting hormones | Calliphora vicina | 3-dehydroecdysone + H2O2 | - |
? | |
| ecdysone + oxidized 2,6-dichloroindophenol | - |
Calliphora vicina | 3-dehydroecdysone + reduced 2,6-dichloroindophenol | - |
r | |
| ecdysteroids + O2 | structural requirements: alpha,beta-unsaturated keto function at C-6 and several hydroxyl functions including the hydroxyls at C-3 and at C-22 | Calliphora vicina | 3-dehydroecdysteroids + H2O2 | - |
? | |
| inokosterone + O2 | - |
Calliphora vicina | 3-dehydroinokosterone + H2O2 | - |
? | |
| makisterone + O2 | - |
Calliphora vicina | 3-dehydromakisterone + H2O2 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Calliphora vicina |
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