Literature summary for 1.1.3.15 extracted from

Lowinsohn, D.; Bertotti, M.
A biosensor based on immobilization of lactate oxidase in a PB-CTAB film for FIA determination of lactate in beer samples (2008), J. Braz. Chem. Soc., 19, 637-642.

No PubMed abstract available

Search for more literature for 1.1.3.15

Application

Application	Comment	Organism
diagnostics	lactate biosensor (FIA amperometric detection), tested with beer samples, detection limit: 0.84 micromol/l, three-electrode cell with AG/AGCl, NaCl reference electrode, platinum wire, glassy carbon electrode, the latter layered with Prussian Blue film of K3Fe(CN)6, Fe(III), KCl, cetyltrimethylammonium bromide, pH 1.7 maintained with HCl, lactate oxidase immobilized onto the Prussian Blue-modified glassy carbon electrode, enzyme layer covered by a Nafion-ethanol solution, storage in phosphate buffer, pH 6.9	Pediococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
lactate + O2	Pediococcus sp.	lactate detection in beer samples	pyruvate + H2O2	H2O2 oxidizes Prussian Blue on an electrode, the concomitant electron flow is measured	?

Organism

Organism	UniProt	Comment	Textmining
Pediococcus sp.	-	-	-

Storage Stability

Storage Stability	Organism
below 0°C, 0.1 mol/l phosphate buffer, pH 7.2 (potassium and sodium salts), 2.2 mg lactate oxidase in 0.5 ml buffer, enzymatic activity stable for several weeks	Pediococcus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
lactate + O2	lactate detection in beer samples	Pediococcus sp.	pyruvate + H2O2	H2O2 oxidizes Prussian Blue on an electrode, the concomitant electron flow is measured	?

Synonyms

Synonyms	Comment	Organism
lactate oxidase	-	Pediococcus sp.
LctO	-	Pediococcus sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.9	-	phosphate buffer, optimal operating conditions	Pediococcus sp.

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Release 2023.1 (January 2023)