Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aerococcus viridans |
Protein Variants | Comment | Organism |
---|---|---|
E160G/V198I | the half-life of the mutant lactate oxidase at 70°C is about 18times that of the wild-type enzyme. Km-value for (S)-lactate at 25°C is 46fold higher than wild-type value, Km-value for (S)-lactate at 25°C is 59fold higher than wild-type value | Aerococcus viridans |
E160G/V198I/G36S/T103S/A232S/F277Y | the half-life of the mutant lactate oxidase at 70°C is about 2 times that of E160G/V198I mutant and about 36 times that of the wild-type enzyme. Km-value for (S)-lactate at 25°C is 48fold higher than wild-type value, Km-value for (S)-lactate at 25°C is 55fold higher than wild-type value. Mutant is obtained by direct evolution | Aerococcus viridans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.529 | - |
(S)-lactate | 25°C, wild-type enzyme | Aerococcus viridans | |
0.863 | - |
(S)-lactate | 35°C, wild-type enzyme | Aerococcus viridans | |
24.3 | - |
(S)-lactate | 25°C, mutant enzyme E160G/V198I | Aerococcus viridans | |
25.5 | - |
(S)-lactate | 25°C, mutant enzyme E160G/V198I/G36S/T103S/A232S/F277Y | Aerococcus viridans | |
47.6 | - |
(S)-lactate | 35°C, mutant enzyme E160G/V198I/G36S/T103S/A232S/F277Y | Aerococcus viridans | |
50.7 | - |
(S)-lactate | 35°C, mutant enzyme E160G/V198I | Aerococcus viridans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aerococcus viridans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + O2 | - |
Aerococcus viridans | acetate + CO2 + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
t1/2: 1.7 min for wild-type enzyme, 30.3 min for mutant enzyme E160G/V198I, 61.4 min for mutant enzyme E160G/V198I/G36S/T103S/A232S/F277Y | Aerococcus viridans |