Protein Variants | Comment | Organism |
---|---|---|
A95G | enhanced activity with bulky substrates, increase in the reduction rate constants with substrates having a bulky alpha-substituent such as mandelate, when compared with wild-type enzyme | Aerococcus viridans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reduction rate constants and dissociation constants for reaction of lactate oxidases with alpha-1H- and alpha-2H-para-substituted mandelates | Aerococcus viridans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aerococcus viridans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-mandelate + O2 | the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive | Aerococcus viridans | phenylglyoxylic acid + H2O2 | - |
? | |
DL-p-hydroxymandelate + O2 | the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive | Aerococcus viridans | ? | - |
? | |
L-4-chloromandelate + O2 | the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive | Aerococcus viridans | ? | - |
? | |
L-4-fluoromandelate + O2 | the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive | Aerococcus viridans | ? | - |
? | |
L-4-methoxymandelate + O2 | the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive | Aerococcus viridans | ? | - |
? | |
L-4-methylmandelate + O2 | the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive | Aerococcus viridans | ? | - |
? | |
L-4-nitromandelate + O2 | the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive | Aerococcus viridans | ? | - |
? | |
L-4-trifluoromethylmandelate + O2 | the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive | Aerococcus viridans | ? | - |
? | |
L-mandelate + O2 | the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive | Aerococcus viridans | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Aerococcus viridans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reduction rate constants and dissociation constants for reaction of lactate oxidases with alpha-1H- and alpha-2H-para-substituted mandelates | Aerococcus viridans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aerococcus viridans |