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Literature summary for 1.1.3.15 extracted from

  • Robinson, J.C.; Keay, L.; Molinari, R.; Sizer, I.W.
    L-alpha-Hydroxy acid oxidases of hog renal cortex (1962), J. Biol. Chem., 237, 2001-2010.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA slight activation for short and long chain oxidases Sus scrofa

General Stability

General Stability Organism
long chain oxidase stable at -20°C, short chain oxidase stable at -20°C for several months Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxybutyrate long chain oxidase, 13-17% inhibition at 17 mM Sus scrofa
4-chloromercuribenzoate long chain oxidase, 29-42% inhibition at 0.001 mM, short chain oxidase: 70-75% inhibition at 0.001 mM Sus scrofa
Atebrin long chain oxidase, 72-76% inhibition at 1 mM, short chain oxidase: 68-76% inhibition at 1 mM Sus scrofa
Cu2+ short chain oxidase: 86% inhibition at 0.1 mM Sus scrofa
DL-beta-Phenyllactate short chain oxidase: 10% inhibition of glycolate oxidation, 81% inhibition of L-2-hydroxisocaproate oxidation at 10 mM Sus scrofa
DL-Lipoate long chain oxidase, 35% inhibition at 0.24 mM, short chain oxidase: 46-52% inhibition at 0.01 mM Sus scrofa
iodoacetate long chain oxidase, 31-36% inhibition at 0.1 mM, short chain oxidase: no inhibition Sus scrofa
L-leucine 9-12% inhibition at 33 mM Sus scrofa
L-phenylalanine long chain oxidase, 43% inhibition at 33 mM Sus scrofa
o-Iodosobenzoate long chain oxidase, 95-100% inhibition at 0.1 mM, short chain oxidase: no inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
glycolate short chain oxidase Sus scrofa
0.68
-
L-2-Hydroxyisocaproate short chain oxidase Sus scrofa
0.68
-
L-2-Hydroxyisocaproate short chain oxidase, at pH 6.0 and 37°C Sus scrofa
2.2
-
L-beta-Phenyllactate long chain oxidase Sus scrofa
2.4
-
L-2-hydroxy-beta-methylvalerate long chain oxidase Sus scrofa
2.5
-
L-2-Hydroxyisocaproate long chain oxidase Sus scrofa
2.5
-
L-2-Hydroxyisocaproate long chain oxidase, at pH 6.0 and 37°C Sus scrofa
16
-
L-lactate short chain oxidase Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Sus scrofa
-
-
mitochondrion
-
Sus scrofa 5739
-

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
pig
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques, 2 distinct forms Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney two forms: long chain oxidase, short chain oxidase Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-2-hydroxy-4-methylmercaptobutyrate + 2,6-dichlorophenolindophenol good substrate for long chain oxidase, low activity for short chain oxidase Sus scrofa 2-oxo-4-methylmercaptobutyrate + reduced 2,6-dichlorophenolindophenol
-
?
DL-2-hydroxybutyrate + 2,6-dichlorophenolindophenol low activity for long chain oxidase, no activity for short chain oxidase Sus scrofa 2-oxobutyrate + reduced 2,6-dichlorophenolindophenol
-
?
DL-2-hydroxycaproate + 2,6-dichlorophenolindophenol low activity for short chain oxidase, moderate activity for long chain oxidase Sus scrofa 2-oxocaproate + reduced 2,6-dichlorophenolindophenol
-
?
DL-2-hydroxydecanoate + 2,6-dichlorophenolindophenol good substrate for long chain oxidase, traces of activity for short chain oxidase Sus scrofa 2-oxodecanoate + reduced 2,6-dichlorophenolindophenol
-
?
DL-2-hydroxyisovalerate + 2,6-dichlorophenolindophenol no activity for short chain oxidase, moderate activity for long chain oxidase Sus scrofa 2-oxoisovalerate + reduced 2,6-dichlorophenolindophenol
-
?
DL-2-hydroxyoctanoate + 2,6-dichlorophenolindophenol good substrate for long chain oxidase, no activity for short chain oxidase Sus scrofa 2-oxooctanoate + reduced 2,6-dichlorophenolindophenol
-
?
DL-2-hydroxyvalerate + 2,6-dichlorophenolindophenol low activity for short chain oxidase, moderate activity for long chain oxidase Sus scrofa 2-oxovalerate + reduced 2,6-dichlorophenolindophenol
-
?
DL-3-indolelactate + 2,6-dichlorophenolindophenol good substrate for long chain oxidase, no activity for short chain oxidase Sus scrofa 3-indolepyruvate + reduced 2,6-dichlorophenolindophenol
-
?
DL-beta-phenyllactate + 2,6-dichlorophenolindophenol no activity for short chain oxidase Sus scrofa phenylpyruvate + reduced 2,6-dichlorophenolindophenol
-
?
DL-mandelate + 2,6-dichlorophenolindophenol low activity Sus scrofa oxo(phenyl)acetic acid + reduced 2,6-dichlorophenolindophenol
-
?
DL-p-hydroxy-beta-phenyllactate + 2,6-dichlorophenolindophenol no activity for short chain oxidase Sus scrofa (4-hydroxyphenyl)pyruvate + reduced 2,6-dichlorophenolindophenol
-
?
glycolate + 2,6-dichlorophenolindophenol highest activity for short chain oxidase, no activity for long chain oxidase Sus scrofa glyoxylate + reduced 2,6-dichlorophenolindophenol
-
?
L-2-hydroxy-beta-methylvalerate + 2,6-dichlorophenolindophenol no activity for short chain oxidase Sus scrofa 3-methyl-2-oxopentanoate + reduced 2,6-dichlorophenolindophenol
-
?
L-2-hydroxyisocaproate + O2 100% activity with long chain oxidase, 32% activity with short chain oxidase Sus scrofa 4-methyl-2-oxopentanoate + H2O2
-
r
L-lactate + 2,6-dichlorophenolindophenol very low activity Sus scrofa pyruvate + reduced 2,6-dichlorophenolindophenol
-
?
L-phenyllactate + 2,6-dichlorophenolindophenol
-
Sus scrofa phenylpyruvate + reduced 2,6-dichlorophenolindophenol
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
short chain oxidase Sus scrofa
7.9
-
long chain oxidase Sus scrofa

Cofactor

Cofactor Comment Organism Structure
FMN
-
Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5
-
Phenyllactate competitive Sus scrofa