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Literature summary for 1.1.3.15 extracted from

  • Nakano, M.; Ushijima, Y.; Saga, M.; Tsutsumi, Y.; Asami, H.
    Aliphatic L-alpha-hydroxyacid oxidase from rat livers. Purification and properties (1968), Biochim. Biophys. Acta, 167, 9-22.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbate 17.5-26% activation at 1 mM Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate strong inhibition at 0.01 mM Rattus sp.
8-hydroxyquinoline 8-17% inhibition at 1 mM Rattus sp.
Cu2+ complete inhibition at 0.025 mM Rattus sp.
diethyldithiocarbamate 51-55% inhibition at 1 mM Rattus sp.
KCN 30% inhibition of L-2-hydroxyisocaproate oxidation at 1 mM, 91% inhibition of glycolate oxidation at 1 mM Rattus sp.
o-Iodosobenzoate 46% inhibition at 0.1 mM Rattus sp.
o-phenanthroline 5-14% inhibition at 1 mM Rattus sp.
Quinacrine 6% inhibition at 1 mM Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
glycolate
-
Rattus sp.
1.34
-
DL-2-hydroxycaproate
-
Rattus sp.
1.65
-
L-2-Hydroxyisocaproate
-
Rattus sp.
8.5
-
L-lactate
-
Rattus sp.
12.7
-
DL-2-hydroxybutyrate
-
Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
gel filtration Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-lactate + O2 Rattus sp. traces of activity pyruvate + H2O2
-
?
DL-2-hydroxybutyrate + O2 Rattus sp.
-
2-oxobutyrate + H2O2
-
?
DL-2-hydroxycaproate + O2 Rattus sp.
-
2-oxocaproate + H2O2
-
?
DL-2-hydroxyisovalerate + O2 Rattus sp. low activity 2-oxoisovalerate + H2O2
-
?
DL-2-hydroxyvalerate + O2 Rattus sp.
-
2-oxovalerate + H2O2
-
?
glycolate + O2 Rattus sp.
-
glyoxylate + H2O2
-
?
L-2-hydroxyisocaproate + O2 Rattus sp. best substrate tested 2-oxoisocaproate + H2O2
-
?
L-lactate + O2 Rattus sp.
-
pyruvate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.43
-
-
Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-lactate + O2 traces of activity Rattus sp. pyruvate + H2O2
-
?
DL-2-hydroxybutyrate + O2
-
Rattus sp. 2-oxobutyrate + H2O2
-
?
DL-2-hydroxycaproate + O2
-
Rattus sp. 2-oxocaproate + H2O2
-
?
DL-2-hydroxyisovalerate + O2 low activity Rattus sp. 2-oxoisovalerate + H2O2
-
?
DL-2-hydroxyvalerate + O2
-
Rattus sp. 2-oxovalerate + H2O2
-
?
glycolate + O2
-
Rattus sp. glyoxylate + H2O2
-
?
L-2-hydroxyisocaproate + O2 best substrate tested Rattus sp. 2-oxoisocaproate + H2O2
-
?
L-lactate + O2
-
Rattus sp. pyruvate + H2O2
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Rattus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.77
-
diethyldithiocarbamate competitive Rattus sp.
0.88
-
KCN uncompetitive Rattus sp.