Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | strong inhibition at 0.01 mM | Rattus sp. | |
8-hydroxyquinoline | 5-17% inhibition at 1 mM | Rattus sp. | |
Cu2+ | nearly complete inhibition at 0.025 mM | Rattus sp. | |
cysteine | 28-38% inhibition of glycolate oxidation at 1 mM | Rattus sp. | |
diethyldithiocarbamate | competitive inhibition | Rattus sp. | |
diphenylglycolic acid | 11-17% inhibition at 52 mM | Rattus sp. | |
hydoxylamine | 66-67% inhibition at 5 mM | Rattus sp. | |
KCN | mixed-type inhibitor | Rattus sp. | |
o-Iodosobenzoate | 46-62% inhibition at 0.1 mM | Rattus sp. | |
o-phenanthroline | 5-23% inhibition at 1 mM | Rattus sp. | |
oxalate | 18% inhibition of glycolate oxidation, 71% inhibition of glyoxylate oxidation at 0.03 mM | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
glycolate | cosubstrates: 2,4-dinitrophenyl hydrazone | Rattus sp. | |
0.24 | - |
glycolate | cosubstrate: O2 | Rattus sp. | |
1.24 | - |
L-2-Hydroxyisocaproate | cosubstrate: 2,4-dinitrophenyl hydrazone | Rattus sp. | |
1.26 | - |
L-2-Hydroxyisocaproate | cosubstrate: O2 | Rattus sp. | |
1.41 | - |
glyoxylate | cosubstrate: O2 | Rattus sp. | |
1.78 | - |
glyoxylate | cosubstrate: 2,4-dinitrophenyl hydrazone | Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Rattus sp. | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-lactate + O2 | Rattus sp. | traces of activity | pyruvate + H2O2 | - |
? | |
DL-2-hydroxybutyrate + O2 | Rattus sp. | - |
2-oxobutyrate + H2O2 | - |
? | |
DL-2-hydroxyisovalerate + O2 | Rattus sp. | low activity | 2-oxoisovalerate + H2O2 | - |
? | |
glycolate + O2 | Rattus sp. | - |
glyoxylate + H2O2 | - |
? | |
glyoxylate + O2 | Rattus sp. | 40% of the activity compared to glycolate | ? + H2O2 | - |
? | |
L-2-hydroxyisocaproate + O2 | Rattus sp. | - |
2-oxoisocaproate + H2O2 | - |
? | |
L-lactate + O2 | Rattus sp. | very low activity | pyruvate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.28 | - |
- |
Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-lactate + O2 | traces of activity | Rattus sp. | pyruvate + H2O2 | - |
? | |
DL-2-hydroxybutyrate + 2,4-dinitrophenylhydrazine | low activity | Rattus sp. | 2-oxobutyrate 2,4-dinitrophenylhydrazone + ? | - |
? | |
DL-2-hydroxybutyrate + O2 | - |
Rattus sp. | 2-oxobutyrate + H2O2 | - |
? | |
DL-2-hydroxyisovalerate + 2,4-dinitrophenylhydrazine | very low activity | Rattus sp. | 2-oxoisovalerate 2,4-dinitrophenylhydrazone + ? | - |
? | |
DL-2-hydroxyisovalerate + O2 | low activity | Rattus sp. | 2-oxoisovalerate + H2O2 | - |
? | |
glycolate + 2,4-dinitrophenylhydrazine | best substrate tested | Rattus sp. | glyoxylate 2,4-dinitrophenylhydrazone + ? | - |
? | |
glycolate + O2 | - |
Rattus sp. | glyoxylate + H2O2 | - |
? | |
glyoxylate + O2 | 40% of the activity compared to glycolate | Rattus sp. | ? + H2O2 | - |
? | |
L-2-hydroxyisocaproate + 2,4-dinitrophenylhydrazine | - |
Rattus sp. | 2-oxoisocaproate 2,4-dinitrophenylhydrazone + ? | - |
? | |
L-2-hydroxyisocaproate + O2 | - |
Rattus sp. | 2-oxoisocaproate + H2O2 | - |
? | |
L-lactate + 2,4-dinitrophenylhydrazine | very low activity | Rattus sp. | pyruvate 2,4-dinitrophenylhydrazone + ? | - |
? | |
L-lactate + O2 | very low activity | Rattus sp. | pyruvate + H2O2 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rattus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Rattus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | 0.39 | diethyldithiocarbamate | competitive inhibition | Rattus sp. | |
0.52 | 0.65 | KCN | mixed-type inhibition | Rattus sp. |