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Literature summary for 1.1.3.15 extracted from

  • Ushijima, Y.
    Identity of aliphatic L-alpha-hydroxyacid oxidase and glycolate oxidase from rat liver (1973), Arch. Biochem. Biophys., 155, 361-367.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate strong inhibition at 0.01 mM Rattus sp.
8-hydroxyquinoline 5-17% inhibition at 1 mM Rattus sp.
Cu2+ nearly complete inhibition at 0.025 mM Rattus sp.
cysteine 28-38% inhibition of glycolate oxidation at 1 mM Rattus sp.
diethyldithiocarbamate competitive inhibition Rattus sp.
diphenylglycolic acid 11-17% inhibition at 52 mM Rattus sp.
hydoxylamine 66-67% inhibition at 5 mM Rattus sp.
KCN mixed-type inhibitor Rattus sp.
o-Iodosobenzoate 46-62% inhibition at 0.1 mM Rattus sp.
o-phenanthroline 5-23% inhibition at 1 mM Rattus sp.
oxalate 18% inhibition of glycolate oxidation, 71% inhibition of glyoxylate oxidation at 0.03 mM Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
glycolate cosubstrates: 2,4-dinitrophenyl hydrazone Rattus sp.
0.24
-
glycolate cosubstrate: O2 Rattus sp.
1.24
-
L-2-Hydroxyisocaproate cosubstrate: 2,4-dinitrophenyl hydrazone Rattus sp.
1.26
-
L-2-Hydroxyisocaproate cosubstrate: O2 Rattus sp.
1.41
-
glyoxylate cosubstrate: O2 Rattus sp.
1.78
-
glyoxylate cosubstrate: 2,4-dinitrophenyl hydrazone Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Rattus sp. 5777
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-lactate + O2 Rattus sp. traces of activity pyruvate + H2O2
-
?
DL-2-hydroxybutyrate + O2 Rattus sp.
-
2-oxobutyrate + H2O2
-
?
DL-2-hydroxyisovalerate + O2 Rattus sp. low activity 2-oxoisovalerate + H2O2
-
?
glycolate + O2 Rattus sp.
-
glyoxylate + H2O2
-
?
glyoxylate + O2 Rattus sp. 40% of the activity compared to glycolate ? + H2O2
-
?
L-2-hydroxyisocaproate + O2 Rattus sp.
-
2-oxoisocaproate + H2O2
-
?
L-lactate + O2 Rattus sp. very low activity pyruvate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.28
-
-
Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-lactate + O2 traces of activity Rattus sp. pyruvate + H2O2
-
?
DL-2-hydroxybutyrate + 2,4-dinitrophenylhydrazine low activity Rattus sp. 2-oxobutyrate 2,4-dinitrophenylhydrazone + ?
-
?
DL-2-hydroxybutyrate + O2
-
Rattus sp. 2-oxobutyrate + H2O2
-
?
DL-2-hydroxyisovalerate + 2,4-dinitrophenylhydrazine very low activity Rattus sp. 2-oxoisovalerate 2,4-dinitrophenylhydrazone + ?
-
?
DL-2-hydroxyisovalerate + O2 low activity Rattus sp. 2-oxoisovalerate + H2O2
-
?
glycolate + 2,4-dinitrophenylhydrazine best substrate tested Rattus sp. glyoxylate 2,4-dinitrophenylhydrazone + ?
-
?
glycolate + O2
-
Rattus sp. glyoxylate + H2O2
-
?
glyoxylate + O2 40% of the activity compared to glycolate Rattus sp. ? + H2O2
-
?
L-2-hydroxyisocaproate + 2,4-dinitrophenylhydrazine
-
Rattus sp. 2-oxoisocaproate 2,4-dinitrophenylhydrazone + ?
-
?
L-2-hydroxyisocaproate + O2
-
Rattus sp. 2-oxoisocaproate + H2O2
-
?
L-lactate + 2,4-dinitrophenylhydrazine very low activity Rattus sp. pyruvate 2,4-dinitrophenylhydrazone + ?
-
?
L-lactate + O2 very low activity Rattus sp. pyruvate + H2O2
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Rattus sp.

Cofactor

Cofactor Comment Organism Structure
FMN
-
Rattus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.27 0.39 diethyldithiocarbamate competitive inhibition Rattus sp.
0.52 0.65 KCN mixed-type inhibition Rattus sp.