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Literature summary for 1.1.3.15 extracted from

  • Cromartie, T.H.; Walsh, C.T.
    Rat kidney L-alpha-hydroxy acid oxidase: Isolation of enzyme with one flavine coenzyme per two subunits (1975), Biochemistry, 14, 2588-2596.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Dithionite reduction of FMN Rattus sp.
L-leucine competitive inhibition Rattus sp.
pyruvate competitive inhibition Rattus sp.
sodium sulfite reduces FMN Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
L-alpha-hydroxyphenyllactate
-
Rattus sp.
3.2
-
DL-2-hydroxycaproate
-
Rattus sp.
8
-
DL-2-hydroxyisovalerate
-
Rattus sp.
13
-
DL-2-hydroxyvalerate
-
Rattus sp.
14
-
DL-2-hydroxybutyrate
-
Rattus sp.
15
-
L-leucine
-
Rattus sp.
27
-
DL-lactate
-
Rattus sp.
28
-
DL-3-chlorolactate
-
Rattus sp.
40
-
L-tryptophan
-
Rattus sp.
53
-
L-methionine
-
Rattus sp.
71
-
DL-alpha-phenyllactate
-
Rattus sp.
90
-
L-lysine
-
Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47500
-
4 * 47500, SDS-PAGE Rattus sp.
190000
-
gel filtration Rattus sp.
250000
-
sedimentation equilibrium analysis Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DL-2-hydroxybutyrate + O2 Rattus sp.
-
2-oxobutyrate + H2O2
-
?
DL-2-hydroxycaproate + O2 Rattus sp.
-
2-oxocaproate + H2O2
-
?
DL-2-hydroxyisovalerate + O2 Rattus sp. low activity 2-oxoisovalerate + H2O2
-
?
DL-2-hydroxyvalerate + O2 Rattus sp.
-
2-oxovalerate + H2O2
-
?
DL-lactate + O2 Rattus sp.
-
pyruvate + H2O2
-
?
glycolate + O2 Rattus sp. preference for long chain substrates, more efficient hydroxy acid oxidase than an amino acid oxidase glyoxylate + H2O2
-
?
L-leucine + O2 Rattus sp.
-
? + H2O2
-
?
L-lysine + O2 Rattus sp.
-
? + H2O2
-
?
L-methionine + O2 Rattus sp.
-
? + H2O2
-
?
L-tryptophan + O2 Rattus sp.
-
? + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.45
-
-
Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-2-hydroxybutyrate + O2
-
Rattus sp. 2-oxobutyrate + H2O2
-
?
DL-2-hydroxycaproate + O2
-
Rattus sp. 2-oxocaproate + H2O2
-
?
DL-2-hydroxyisovalerate + O2 low activity Rattus sp. 2-oxoisovalerate + H2O2
-
?
DL-2-hydroxyvalerate + O2
-
Rattus sp. 2-oxovalerate + H2O2
-
?
DL-3-chlorolactate + O2 best substrate tested Rattus sp. 3-chloropyruvate + H2O2
-
?
DL-alpha-phenyllactate + O2
-
Rattus sp. ? + H2O2
-
r
DL-lactate + O2
-
Rattus sp. pyruvate + H2O2
-
?
DL-phenyllactate + O2
-
Rattus sp. phenylpyruvate + H2O2
-
?
glycolate + O2 preference for long chain substrates, more efficient hydroxy acid oxidase than an amino acid oxidase Rattus sp. glyoxylate + H2O2
-
?
L-2-hydroxyphenyllactate + O2
-
Rattus sp. ? + H2O2
-
?
L-alpha-hydroxyphenyllactate + O2
-
Rattus sp. ? + H2O2
-
r
L-leucine + O2
-
Rattus sp. ? + H2O2
-
?
L-lysine + O2
-
Rattus sp. ? + H2O2
-
?
L-methionine + O2
-
Rattus sp. ? + H2O2
-
?
L-tryptophan + O2
-
Rattus sp. ? + H2O2
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 47500, SDS-PAGE Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
Rattus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
half-maximum oxidation rate Rattus sp.
9
-
half-maximum oxidation rate Rattus sp.

Cofactor

Cofactor Comment Organism Structure
FMN one FAD per two subunits Rattus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
23
-
L-leucine
-
Rattus sp.