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Literature summary for 1.1.3.15 extracted from

  • Duley, J.A.; Holmes, R.S.
    L-alpha-hydroxyacid oxidase isozymes. Purification and properties (1976), Eur. J. Biochem., 63, 163-173.
    View publication on PubMed
Search for more literature for 1.1.3.15

General Stability

General Stability Organism
loss of activity due to loss of irreversibly removed FMN during storage Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, isoenzymes A and B, SDS-PAGE Rattus sp.
40000
-
 x * 40000, isoenzymes A and B, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycolate + O2 Mus musculus substrate for isoenzyme A glyoxylate + H2O2
-
 ?
glycolate + O2 Rattus sp. substrate for isoenzyme A glyoxylate + H2O2
-
 ?
L-2-hydroxyisocaproate + O2 Mus musculus substrate for isoenzyme B 2-oxoisocaproate + H2O2
-
 ?
L-2-hydroxyisocaproate + O2 Rattus sp. substrate for isoenzyme B 2-oxoisocaproate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, isoenzymes A and B Mus musculus
to homogeneity, chromatography steps Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Mus musculus
-
kidney isoenzyme L-2-hydroxyacid oxidase B Rattus sp.
-
liver
-
 Mus musculus
-
liver isoenzyme L-2-hydroxyacid oxidase A Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.88
-
 isoenzyme B Rattus sp.
3.66
-
 isoemzyme A Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycolate + O2 substrate for isoenzyme A Mus musculus glyoxylate + H2O2
-
 ?
glycolate + O2 substrate for isoenzyme A Rattus sp. glyoxylate + H2O2
-
 ?
L-2-hydroxyisocaproate + O2 substrate for isoenzyme B Mus musculus 2-oxoisocaproate + H2O2
-
 ?
L-2-hydroxyisocaproate + O2 substrate for isoenzyme B Rattus sp. 2-oxoisocaproate + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, isoenzymes A and B, SDS-PAGE Rattus sp.
? x * 40000, isoenzymes A and B, SDS-PAGE Mus musculus

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Mus musculus
FMN one FAD per subunit Rattus sp.