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Literature summary for 1.1.3.13 extracted from

  • Mangkorn, N.; Kanokratana, P.; Roongsawang, N.; Laobuthee, A.; Laosiripojana, N.; Champreda, V.
    Synthesis and characterization of Ogataea thermomethanolica alcohol oxidase immobilized on barium ferrite magnetic microparticles (2019), J. Biosci. Bioeng., 127, 265-272 .
    View publication on PubMed
Search for more literature for 1.1.3.13

Application

Application Comment Organism
synthesis when covalently immobilized onto barium ferrite (BaFe12O19) magnetic microparticles, an immobilization efficiency of 71.0 % and catalytic activity of 34.6 U/g can be obtained. The immobilized OthAOX works optimally at 55°C and pH 8.0. More than 65% of the initial immobilized enzyme activity is retained after 24 h pre-incubation at 45°C. The immobilized enzyme shows a greater catalytic efficiency for oxidation of methanol and ethanol than free enzyme and can be recovered by magnetization and recycled for at least three consecutive batches, after which 70% activity remains Ogataea thermomethanolica (nom. inval.)

Organism

Organism UniProt Comment Textmining
Ogataea thermomethanolica (nom. inval.) A0A067YIW8
-
-

Purification (Commentary)

Purification (Comment) Organism
using a DEAE-sepharose column Ogataea thermomethanolica (nom. inval.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + O2
-
 Ogataea thermomethanolica (nom. inval.) butanal + H2O2
-
 ?
1-pentanol + O2
-
 Ogataea thermomethanolica (nom. inval.) pentanal + H2O2
-
 ?
1-propanol + O2
-
 Ogataea thermomethanolica (nom. inval.) propanal + H2O2
-
 ?
ethanol + O2
-
 Ogataea thermomethanolica (nom. inval.) acetaldehyde + H2O2
-
 ?
methanol + O2
-
 Ogataea thermomethanolica (nom. inval.) formaldehyde + H2O2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 immobilized enzyme Ogataea thermomethanolica (nom. inval.)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
 60% of maximum activity, immobilized enzyme Ogataea thermomethanolica (nom. inval.)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 24 h, more than 65% reisudal activity, immobilized enzyme. The free AOX retains only 15% activity Ogataea thermomethanolica (nom. inval.)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 immobilized enzyme Ogataea thermomethanolica (nom. inval.)

pH Range

pH Minimum pH Maximum Comment Organism
6 9 more than 75% of maximum activity, immobilized enzyme Ogataea thermomethanolica (nom. inval.)