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Literature summary for 1.1.3.13 extracted from

  • Yamada, M.; Higashiyama, T.; Kishino, S.; Kataoka, M.; Ogawa, J.; Shimizu, S.; Isobe, K.
    Novel alcohol oxidase with glycolate oxidase activity from Ochrobactrum sp. AIU 033 (2014), J. Mol. Catal. B, 105, 41-48.
No PubMed abstract available
Search for more literature for 1.1.3.13

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 91% residual activity at 1 mM Ochrobactrum sp.
EDTA 91% residual activity at 1 mM Ochrobactrum sp.
hydrazine 89% residual activity at 1 mM Ochrobactrum sp.
hydroxylamine 72% residual activity at 1 mM Ochrobactrum sp.
iodoacetic acid 59% residual activity at 1 mM Ochrobactrum sp.
KCN 93% residual activity at 1 mM Ochrobactrum sp.
Mg2+ 93% residual activity at 1 mM Ochrobactrum sp.
N-ethylmaleimide 84% residual activity at 1 mM Ochrobactrum sp.
o-phenanthroline 93% residual activity at 1 mM Ochrobactrum sp.
phenylhydrazine 1% residual activity at 1 mM Ochrobactrum sp.
Semicarbazide 91% residual activity at 1 mM Ochrobactrum sp.
Sodium azide 97% residual activity at 1 mM Ochrobactrum sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0109
-
 1-Decanol at pH 5.5 and 30°C Ochrobactrum sp.
0.0365
-
 1-Octanol at pH 5.5 and 30°C Ochrobactrum sp.
0.41
-
 1-Hexanol at pH 5.5 and 30°C Ochrobactrum sp.
5.54
-
 1-Pentanol at pH 5.5 and 30°C Ochrobactrum sp.
15.8
-
 Lactate at pH 5.5 and 30°C Ochrobactrum sp.
167
-
 glycolate at pH 5.5 and 30°C Ochrobactrum sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ the enzyme contains 2 iron atoms and shows 112% activity at 1 mM Fe3+ Ochrobactrum sp.
additional information the enzyme is not influenced by Mn2+, Co2+, Ni2+, and Zn2+ Ochrobactrum sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
-
 Ochrobactrum sp.
52000
-
-
 Ochrobactrum sp.
130000
-
 gel filtration Ochrobactrum sp.

Organism

Organism UniProt Comment Textmining
Ochrobactrum sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl-Toyopearl 650 M resin column chromatography, Q-Toyopearl 650 M column chromatography, and hydroxyapatite column chromatography Ochrobactrum sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0407
-
 cell-free extract, with glycolate as substrate, at pH 5.5 and 30°C Ochrobactrum sp.
4.02
-
 after 101fold purification, with glycolate as substrate, at pH 5.5 and 30°C Ochrobactrum sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + O2 373% activity compared to glycolate Ochrobactrum sp. butanal + H2O2
-
 ?
1-decanol + O2 180% activity compared to glycolate Ochrobactrum sp. decanal + H2O2
-
 ?
1-hexanol + O2 300% activity compared to glycolate Ochrobactrum sp. hexanal + H2O2
-
 ?
1-octanol + O2 189% activity compared to glycolate Ochrobactrum sp. octanal + H2O2
-
 ?
1-pentanol + O2 448% activity compared to glycolate Ochrobactrum sp. pentanal + H2O2
-
 ?
1-propanol + O2 63% activity compared to glycolate Ochrobactrum sp. propanal + H2O2
-
 ?
ethanol + O2 10% activity compared to glycolate Ochrobactrum sp. acetaldehyde + H2O2
-
 ?
glycolate + O2 100% activity Ochrobactrum sp. glyoxylate + H2O2
-
 ?
lactate + O2 14% activity compared to glycolate Ochrobactrum sp. decanal + H2O2
-
 ?
additional information the enzyme does not oxidize benzyl alcohol, glycolaldehyde, glyoxylate, ethylene glycol, glycerol, or methanol Ochrobactrum sp. ?
-
 ?

Subunits

Subunits Comment Organism
heterotetramer 2 * 52000 + 2 * 14000, SDS-PAGE Ochrobactrum sp.

Synonyms

Synonyms Comment Organism
AOX
-
 Ochrobactrum sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Ochrobactrum sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 the enzyme exhibits more than 60% activity between 25 and 45°C, and no activity at 50°C Ochrobactrum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Ochrobactrum sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6.5 more than 80% of the enzyme activity remains in the pH range from 5.5 to 6.5 Ochrobactrum sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Ochrobactrum sp.