Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 91% residual activity at 1 mM | Ochrobactrum sp. | |
EDTA | 91% residual activity at 1 mM | Ochrobactrum sp. | |
hydrazine | 89% residual activity at 1 mM | Ochrobactrum sp. | |
hydroxylamine | 72% residual activity at 1 mM | Ochrobactrum sp. | |
iodoacetic acid | 59% residual activity at 1 mM | Ochrobactrum sp. | |
KCN | 93% residual activity at 1 mM | Ochrobactrum sp. | |
Mg2+ | 93% residual activity at 1 mM | Ochrobactrum sp. | |
N-ethylmaleimide | 84% residual activity at 1 mM | Ochrobactrum sp. | |
o-phenanthroline | 93% residual activity at 1 mM | Ochrobactrum sp. | |
phenylhydrazine | 1% residual activity at 1 mM | Ochrobactrum sp. | |
Semicarbazide | 91% residual activity at 1 mM | Ochrobactrum sp. | |
Sodium azide | 97% residual activity at 1 mM | Ochrobactrum sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0109 | - |
1-Decanol | at pH 5.5 and 30°C | Ochrobactrum sp. | |
0.0365 | - |
1-Octanol | at pH 5.5 and 30°C | Ochrobactrum sp. | |
0.41 | - |
1-Hexanol | at pH 5.5 and 30°C | Ochrobactrum sp. | |
5.54 | - |
1-Pentanol | at pH 5.5 and 30°C | Ochrobactrum sp. | |
15.8 | - |
Lactate | at pH 5.5 and 30°C | Ochrobactrum sp. | |
167 | - |
glycolate | at pH 5.5 and 30°C | Ochrobactrum sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | the enzyme contains 2 iron atoms and shows 112% activity at 1 mM Fe3+ | Ochrobactrum sp. | |
additional information | the enzyme is not influenced by Mn2+, Co2+, Ni2+, and Zn2+ | Ochrobactrum sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
- |
Ochrobactrum sp. |
52000 | - |
- |
Ochrobactrum sp. |
130000 | - |
gel filtration | Ochrobactrum sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ochrobactrum sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl-Toyopearl 650 M resin column chromatography, Q-Toyopearl 650 M column chromatography, and hydroxyapatite column chromatography | Ochrobactrum sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0407 | - |
cell-free extract, with glycolate as substrate, at pH 5.5 and 30°C | Ochrobactrum sp. |
4.02 | - |
after 101fold purification, with glycolate as substrate, at pH 5.5 and 30°C | Ochrobactrum sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + O2 | 373% activity compared to glycolate | Ochrobactrum sp. | butanal + H2O2 | - |
? | |
1-decanol + O2 | 180% activity compared to glycolate | Ochrobactrum sp. | decanal + H2O2 | - |
? | |
1-hexanol + O2 | 300% activity compared to glycolate | Ochrobactrum sp. | hexanal + H2O2 | - |
? | |
1-octanol + O2 | 189% activity compared to glycolate | Ochrobactrum sp. | octanal + H2O2 | - |
? | |
1-pentanol + O2 | 448% activity compared to glycolate | Ochrobactrum sp. | pentanal + H2O2 | - |
? | |
1-propanol + O2 | 63% activity compared to glycolate | Ochrobactrum sp. | propanal + H2O2 | - |
? | |
ethanol + O2 | 10% activity compared to glycolate | Ochrobactrum sp. | acetaldehyde + H2O2 | - |
? | |
glycolate + O2 | 100% activity | Ochrobactrum sp. | glyoxylate + H2O2 | - |
? | |
lactate + O2 | 14% activity compared to glycolate | Ochrobactrum sp. | decanal + H2O2 | - |
? | |
additional information | the enzyme does not oxidize benzyl alcohol, glycolaldehyde, glyoxylate, ethylene glycol, glycerol, or methanol | Ochrobactrum sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 52000 + 2 * 14000, SDS-PAGE | Ochrobactrum sp. |
Synonyms | Comment | Organism |
---|---|---|
AOX | - |
Ochrobactrum sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Ochrobactrum sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | the enzyme exhibits more than 60% activity between 25 and 45°C, and no activity at 50°C | Ochrobactrum sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Ochrobactrum sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | more than 80% of the enzyme activity remains in the pH range from 5.5 to 6.5 | Ochrobactrum sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Ochrobactrum sp. |