Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ferrocene | 1 mM, increases the alcohol oxidase activity only for the short chain alcohol substrate to 47% of the original activity | Aspergillus terreus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,12-dichlorododecane | - |
Aspergillus terreus | |
2-mercaptoethanol | - |
Aspergillus terreus | |
AgNO3 | 1 mM, complete loss of activity | Aspergillus terreus | |
PCMB | 5 mM, complete loss of activity | Aspergillus terreus | |
Tetraethylthiuram disulfide | 5 mM, more than 70% loss of activity | Aspergillus terreus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.498 | - |
n-Heptanol | - |
Aspergillus terreus | |
1.86 | - |
n-dodecanol | - |
Aspergillus terreus | |
2.3 | - |
phenyl-3-propanol | - |
Aspergillus terreus | |
18.3 | - |
(R)-2-octanol | - |
Aspergillus terreus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Aspergillus terreus | 16020 | - |
microsome | - |
Aspergillus terreus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | 1 mM, increases the enzyme activity to nearly 50% for both short chain-, and long chain alcohol substrates | Aspergillus terreus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13000 | - |
x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE | Aspergillus terreus |
27000 | - |
x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE | Aspergillus terreus |
43000 | - |
x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE | Aspergillus terreus |
63000 | - |
x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE | Aspergillus terreus |
85000 | - |
x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE | Aspergillus terreus |
269000 | - |
non-denaturing PAGE | Aspergillus terreus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | - |
MTCC 6324 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | presence of oleic acid and palmitic acid at a ratio of 2:1 in the purified protein. This lipoidic nature of the protein particles is correlated to the high aggregating property | Aspergillus terreus |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus terreus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:n-hexadecane grown-cell | - |
Aspergillus terreus | - |
Storage Stability | Organism |
---|---|
4°C, 1 month, more than 50% of the activity is retained | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-octanol + O2 | - |
Aspergillus terreus | 2-octanone + H2O2 | - |
? | |
n-dodecanol + O2 | - |
Aspergillus terreus | n-dodecanal + H2O2 | - |
? | |
n-heptanol + O2 | substrates with highest affinity | Aspergillus terreus | n-heptanal + H2O2 | - |
? | |
phenyl-3-propanol + O2 | - |
Aspergillus terreus | 3-phenylpropanal | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 85000 + x * 63000 + x * 43000 + x * 27000 + x * 13000, SDS-PAGE | Aspergillus terreus |
Synonyms | Comment | Organism |
---|---|---|
broad substrate specific alcohol oxidase | - |
Aspergillus terreus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
1 month, more than 50% of the activity is retained | Aspergillus terreus |
32 | - |
2 h, 80% loss of activity | Aspergillus terreus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
270 | - |
n-Heptanol | - |
Aspergillus terreus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
3 h, 5% loss of activity | Aspergillus terreus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavin is non-covalently but avidly associated. Peptide mass fingerprinting studies show the presence of two FAD binding domains in 63 kDa protein | Aspergillus terreus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000014 | - |
2-mercaptoethanol | substrate: R-(-)-2-octanol | Aspergillus terreus | |
0.00063 | - |
2-mercaptoethanol | substrate: n-dodecanol | Aspergillus terreus | |
0.00157 | - |
2-mercaptoethanol | substrate: n-heptanol | Aspergillus terreus | |
0.00246 | - |
1,12-dichlorododecane | substrate: n-dodecanal | Aspergillus terreus | |
0.00362 | - |
2-mercaptoethanol | substrate: phenyl-3-propanol | Aspergillus terreus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus terreus | isoelectric focusing, pH-range: 3-10 | 8.5 | 8.3 |