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Literature summary for 1.1.3.13 extracted from

  • Suye, S.
    Purification and properties of alcohol oxidase from Candida methanosorbosa M-2003 (1997), Curr. Microbiol., 34, 374-377.
    View publication on PubMed
Search for more literature for 1.1.3.13

General Stability

General Stability Organism
7.7 mM NaN3, 30°C, enzyme retains activity for at least 35 days, inactivated completely after 10 days without NaN3 [Candida] methanosorbosa

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM CuSO4, 30 min, strong [Candida] methanosorbosa
Hg2+ 1 mM HgCl2, 30 min, strong [Candida] methanosorbosa
hydroquinone 1 mM, 30 min, strong [Candida] methanosorbosa
NEM 1 mM, 30 min, strong [Candida] methanosorbosa
PCMB 1 mM, 30 min, strong [Candida] methanosorbosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.43
-
 methanol
-
 [Candida] methanosorbosa
8.23
-
 ethanol
-
 [Candida] methanosorbosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 8 * 80000, SDS-PAGE [Candida] methanosorbosa
620000
-
 gel filtration [Candida] methanosorbosa

Organism

Organism UniProt Comment Textmining
[Candida] methanosorbosa
-
 M-2003
-
[Candida] methanosorbosa M-2003
-
 M-2003
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Candida] methanosorbosa

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 [Candida] methanosorbosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.11
-
-
 [Candida] methanosorbosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-octanol + O2 2.4% of the activity with methanol [Candida] methanosorbosa n-octanal + H2O2
-
 ?
1-pentanol + O2 33.5% of the activity with methanol [Candida] methanosorbosa n-pentanal + H2O2
-
 ?
1-pentanol + O2 33.5% of the activity with methanol [Candida] methanosorbosa M-2003 n-pentanal + H2O2
-
 ?
2-butanol + O2 3.5% of the activity with methanol [Candida] methanosorbosa 2-butanone + H2O2
-
 ?
2-butanol + O2 3.5% of the activity with methanol [Candida] methanosorbosa M-2003 2-butanone + H2O2
-
 ?
benzyl alcohol + O2 21.3% of the activity with methanol [Candida] methanosorbosa benzaldehyde + H2O2
-
 ?
benzyl alcohol + O2 21.3% of the activity with methanol [Candida] methanosorbosa M-2003 benzaldehyde + H2O2
-
 ?
ethanol + O2 97.8% of the activity with methanol [Candida] methanosorbosa acetaldehyde + H2O2
-
 ?
ethanol + O2 97.8% of the activity with methanol [Candida] methanosorbosa M-2003 acetaldehyde + H2O2
-
 ?
methanol + O2
-
 [Candida] methanosorbosa formaldehyde + H2O2
-
 ?
methanol + O2
-
 [Candida] methanosorbosa M-2003 formaldehyde + H2O2
-
 ?
n-butanol + O2 52.2% of the activity with methanol [Candida] methanosorbosa n-butanal + H2O2
-
 ?
n-hexanol + O2 6.3% of the activity with methanol [Candida] methanosorbosa n-hexanal + H2O2
-
 ?
n-propanol + O2 77.5% of the activity with methanol [Candida] methanosorbosa n-propanal + H2O2
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 80000, SDS-PAGE [Candida] methanosorbosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 [Candida] methanosorbosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 6.0-8.5. 60 min, stable [Candida] methanosorbosa
50
-
 pH 7.5, 15 min, stable below [Candida] methanosorbosa
55
-
 15 min, 34% loss of activity [Candida] methanosorbosa
65
-
 15 min, complete inactivation above [Candida] methanosorbosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9
-
 [Candida] methanosorbosa

Cofactor

Cofactor Comment Organism Structure
FAD
-
 [Candida] methanosorbosa