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Literature summary for 1.1.3.13 extracted from

  • Patel, R.N.; Hou, C.T.; Laskin, A.I.; Derelanko, P.
    Microbial oxidation of methanol: properties of crystallized alcohol oxidase from a yeast, Pichia sp (1981), Arch. Biochem. Biophys., 210, 481-488.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Pichia sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 10% inhibition Pichia sp.
2,2'-dipyridyl 1 mM, 13% inhibition Pichia sp.
2-Aminoethanol 1 mM, 70% inhibition Pichia sp.
3-Pentanol 1 mM, 16% inhibition Pichia sp.
5,5'-dithiobis(2-nitrobenzoate) 1 mM, 35% inhibition Pichia sp.
8-hydroxyquinoline 1 mM, 6% inhibition Pichia sp.
acetamide 1 mM, 10% inhibition Pichia sp.
Cu2+ 1 mM CuSO4, 50% inhibition Pichia sp.
H2O2
-
Pichia sp.
hydroxylamine 1 mM, 21% inhibition Pichia sp.
imidazole 1 mM, 20% inhibition Pichia sp.
iodoacetate 1 mM, 38% inhibition Pichia sp.
Mercuric chloride 1 mM, complete inhibition Pichia sp.
metal chelators slight Pichia sp.
NEM 1 mM, 10% inhibition Pichia sp.
p-hydroxymercuribenzoate 1 mM, complete inhibition Pichia sp.
PCMB
-
Pichia sp.
Sodium azide 1 mM, 15% inhibition Pichia sp.
Thiosemicarbazide 1 mM, 31% loss of activity Pichia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
methanol
-
Pichia sp.
3.5
-
formaldehyde
-
Pichia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
4 * 76000, SDS-PAGE Pichia sp.
300000
-
gel filtration Pichia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + O2 Pichia sp. key enzyme of methanol metabolism formaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Pichia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pichia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.6
-
-
Pichia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pentanol + O2 30% of the activity with methanol Pichia sp. n-pentanal + H2O2
-
?
2-mercaptoethanol + O2 25% of the activity with methanol Pichia sp. 2-mercaptoethanal + H2O2
-
?
2-methyl-1-butanol + O2 22% of the activity with methanol Pichia sp. 2-methyl-1-butanal + H2O2
-
?
3-chloro-1-propanol + O2 22% of the activity with methanol Pichia sp. 3-chloro-1-propanal + H2O2
-
?
4-chloro-1-butanol + O2 11% of the activity with methanol Pichia sp. 4-chloro-1-butanal + H2O2
-
?
chloroethanol + O2 2-chloroethanol, 70% of the activity with methanol Pichia sp. chloroethanal + H2O2
-
?
ethanol + O2 92% of the activity with methanol Pichia sp. acetaldehyde + H2O2
-
?
ethylene glycol mono-methylether + O2
-
Pichia sp. methoxyacetaldehyde + H2O2
-
?
formaldehyde + O2 15% of the activity with methanol Pichia sp. formate + H2O2
-
?
isobutanol + O2 2% of the activity with methanol Pichia sp. isobutanal + H2O2
-
?
methanol + O2
-
Pichia sp. formaldehyde + H2O2
-
?
methanol + O2 key enzyme of methanol metabolism Pichia sp. formaldehyde + H2O2
-
?
n-butanol + O2 52%, of the activity with methanol Pichia sp. n-butanal + H2O2
-
?
n-hexanol + O2 4% of the activity with methanol Pichia sp. n-hexanal + H2O2
-
?
n-propanol + O2
-
Pichia sp. n-propanal + H2O2
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 76000, SDS-PAGE Pichia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Pichia sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0: about 65% of maximal activity, pH 10.0: 85% of maximal activity Pichia sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pichia sp.