General Stability | Organism |
---|---|
freezing at -20°C and thawing leads to 25% loss of activity | Poria contigua |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaN3 | strong | Poria contigua | |
PCMB | 1 mM, complete inhibition | Poria contigua |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
methanol | - |
Poria contigua | |
1 | - |
ethanol | - |
Poria contigua | |
3.2 | - |
2-propin-1-ol | - |
Poria contigua | |
6.1 | - |
formaldehyde | - |
Poria contigua | |
8.3 | - |
n-Propanol | - |
Poria contigua | |
21.3 | - |
n-butanol | - |
Poria contigua | |
25 | - |
Isopropanol | - |
Poria contigua | |
27.2 | - |
2-mercaptoethanol | - |
Poria contigua |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Poria contigua | 5737 | - |
microbody | - |
Poria contigua | 42579 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no iron cofactor | Poria contigua |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
8 * 79000, SDS-PAGE | Poria contigua |
610000 | - |
equilibrium sedimentation | Poria contigua |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + O2 | Poria contigua | key enzyme of methanol metabolism | formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Poria contigua | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Poria contigua |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Poria contigua | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.7 | - |
- |
Poria contigua |
Storage Stability | Organism |
---|---|
4°C, purified, stable for 2 weeks | Poria contigua |
4°C, storage in presence of antimicrobial agent sodium azide, 0.01% in 50 mM potassium phosphate buffer, pH 7.7, stable for at least 2 weeks | Poria contigua |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-mercaptoethanol + O2 | - |
Poria contigua | 2-mercaptoethanal + H2O2 | - |
? | |
2-propyn-1-ol + O2 | - |
Poria contigua | propyn-1-al + H2O2 | - |
? | |
2-propyn-1-ol + O2 | - |
Poria contigua | 2-propyn-1-al + H2O2 | - |
? | |
ethanol + O2 | - |
Poria contigua | acetaldehyde + H2O2 | - |
? | |
formaldehyde + O2 | - |
Poria contigua | formate + H2O2 | - |
? | |
isopropanol + O2 | - |
Poria contigua | 2-propanone + H2O2 | - |
? | |
methanol + O2 | - |
Poria contigua | formaldehyde + H2O2 | - |
? | |
methanol + O2 | key enzyme of methanol metabolism | Poria contigua | formaldehyde + H2O2 | - |
? | |
n-butanol + O2 | - |
Poria contigua | n-butanal + H2O2 | - |
? | |
n-propanol + O2 | - |
Poria contigua | n-propanal + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 79000, SDS-PAGE | Poria contigua |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol FAD per mol of subunit | Poria contigua |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Sodium azide | - |
Poria contigua |