Literature summary for 1.1.3.12 extracted from

  • Yuan, B.; Yoshikane, Y.; Yokochi, N.; Ohnishi, K.; Yagi, T.
    The nitrogen-fixing symbiotic bacterium Mesorhizobium loti has and expresses the gene encoding pyridoxine 4-oxidase involved in the degradation of vitamin B6 (2004), FEMS Microbiol. Lett., 234, 225-230.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
O2 pH 8.0, 30°C Mesorhizobium loti
0.213
-
pyridoxine pH 8.0, 30°C Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
1 * 53000, SDS-PAGE, 1 * 55000, deduced from gene sequence Mesorhizobium loti
53000
-
1 * 53000, SDS-PAGE, 1 * 55000, deduced from gene sequence Mesorhizobium loti
54500
-
gel filtration Mesorhizobium loti

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mesorhizobium loti

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.4
-
pH 8.0, 30°C Mesorhizobium loti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates are: pyridoxamine, pyridoxal 5’-phosphate, pyridoxamine 5’-phosphate, benzyl alcohol, 2-hydroxybenzyl alcohol Mesorhizobium loti ?
-
?
pyridoxine + O2
-
Mesorhizobium loti pyridoxal + H2O2
-
?
2,6-dihydroxypyridine + O2 0.04% of the rate with pyridoxine Mesorhizobium loti ? + H2O2
-
?
4-pyridinemethanol + O2 0.12% of the rate with pyridoxine Mesorhizobium loti isonicotinaldehyde + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 53000, SDS-PAGE, 1 * 55000, deduced from gene sequence Mesorhizobium loti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Mesorhizobium loti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Mesorhizobium loti

Cofactor

Cofactor Comment Organism Structure
FAD
-
Mesorhizobium loti