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Literature summary for 1.1.3.11 extracted from
- Enzymatic studies on the oxidation of sugar and sugar alcohol. 3. Purification and properties of L-sorbose oxidase from Trametes sanguinea (1967), J. Biochem., 62, 223-229.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 0.1 mM AgNO3 complete inhibition | Trametes sanguinea |  |
| Cu2+ | slight | Trametes sanguinea | |
| F- | slight | Trametes sanguinea | |
| Hg2+ | 0.1 mM HgCl2 complete inhibition | Trametes sanguinea | |
| Mn2+ | slight | Trametes sanguinea | |
| Ni2+ | slight | Trametes sanguinea | |
| Pb2+ | slight | Trametes sanguinea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
D-glucose | acceptor O2 | Trametes sanguinea | |
| 1.8 | - |
D-glucose | acceptor 2,6-dichlorophenolindophenol | Trametes sanguinea | |
| 22 | - |
L-sorbose | acceptor O2 | Trametes sanguinea | |
| 100 | - |
L-sorbose | acceptor 2,6-dichlorophenolindophenol | Trametes sanguinea | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-sorbose + O2 | Trametes sanguinea | - |
5-dehydro-D-fructose + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes sanguinea | - |
IFO 4923 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trametes sanguinea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Trametes sanguinea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose + O2 | - |
Trametes sanguinea | 2-dehydro-D-galactose + H2O2 | - |
? | |
| D-glucose + O2 | - |
Trametes sanguinea | 2-dehydro-D-glucose + H2O2 | - |
? | |
| D-xylose + O2 | - |
Trametes sanguinea | ? | - |
? | |
| L-sorbose + 2,6-dichloroindophenol | - |
Trametes sanguinea | 5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| L-sorbose + O2 | - |
Trametes sanguinea | 5-dehydro-D-fructose + H2O2 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 60 | 5 min, stable | Trametes sanguinea |
| 75 | - |
5 min, complete loss of activity | Trametes sanguinea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 6 | potassium phosphate buffer | Trametes sanguinea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.1 | 7 | pH 5.1: about 85% of maximal activity, pH 7.0: about 40% of maximal activity | Trametes sanguinea |
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