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Literature summary for 1.1.3.10 extracted from
- Immobilization of pyranose oxidase (Phanerochaete chrysosporium) characterization of the enzymic properties (1991), Enzyme Microb. Technol., 13, 755-759 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | immobilization of enzyme to a glass-beaded support with activity yields of 10%-23.3%. after 4,800 measurement cycles carried out over 5 days, 58-62% of activity remains | Phanerodontia chrysosporium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.58 | - |
D-glucose | immobilized enzyme, pH 6.5, 25°C | Phanerodontia chrysosporium |  |
| 0.93 | - |
D-glucose | native enzyme, pH 6.5, 25°C | Phanerodontia chrysosporium | |
| 6.3 | - |
D-xylose | immobilized enzyme, pH 6.5, 25°C | Phanerodontia chrysosporium | |
| 18.4 | - |
D-xylose | native enzyme, pH 6.5, 25°C | Phanerodontia chrysosporium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | Q6QWR1 | - |
- |
| Phanerodontia chrysosporium K-3 | Q6QWR1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phanerodontia chrysosporium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, half-life of native enzyme is 5 weeks, half-life of immobilized enzyme is 11 weeks | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucono-1,5-lactone + 3-methyl-2-benzothiazolinone hydrazone | - |
Phanerodontia chrysosporium | 2-dehydro-D-glucono-1,5-lactone | - |
? | |
| D-glucono-1,5-lactone + 3-methyl-2-benzothiazolinone hydrazone | - |
Phanerodontia chrysosporium K-3 | 2-dehydro-D-glucono-1,5-lactone | - |
? | |
| D-glucose + 3-methyl-2-benzothiazolinone hydrazone | - |
Phanerodontia chrysosporium | 2-dehydro-D-glucose + ? | - |
? | |
| D-glucose + 3-methyl-2-benzothiazolinone hydrazone | - |
Phanerodontia chrysosporium K-3 | 2-dehydro-D-glucose + ? | - |
? | |
| D-xylose + 3-methyl-2-benzothiazolinone hydrazone | - |
Phanerodontia chrysosporium | 2-dehydro-D-xylose + ? | - |
? | |
| D-xylose + 3-methyl-2-benzothiazolinone hydrazone | - |
Phanerodontia chrysosporium K-3 | 2-dehydro-D-xylose + ? | - |
? | |
| L-sorbose + 3-methyl-2-benzothiazolinone hydrazone | - |
Phanerodontia chrysosporium | ? | - |
? | |
| L-sorbose + 3-methyl-2-benzothiazolinone hydrazone | - |
Phanerodontia chrysosporium K-3 | ? | - |
? | |
| additional information | at 10 mM concentration, glucose is the best substrate, but xylose, sorbose, and D-glucono-1,5-lactone have more than half of its activity | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | at 10 mM concentration, glucose is the best substrate, but xylose, sorbose, and D-glucono-1,5-lactone have more than half of its activity | Phanerodontia chrysosporium K-3 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| P2Ox | - |
Phanerodontia chrysosporium |
| PROD | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 43 | - |
both native and immobilized enzyme | Phanerodontia chrysosporium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
native enzyme | Phanerodontia chrysosporium |
| 8 | - |
immobilized enzyme | Phanerodontia chrysosporium |
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