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Literature summary for 1.1.3.10 extracted from

  • Ai, M.; Wang, F.; Zhang, Y.; Huang, F.
    Purification of pyranose oxidase from the white rot fungus Irpex lacteus and its cooperation with laccase in lignin degradation (2014), Process Biochem., 49, 2191-2198.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.74
-
D-glucose at pH 6.5 and 25°C Irpex lacteus
7.73
-
D-galactose at pH 6.5 and 25°C Irpex lacteus
25.9
-
D-xylose at pH 6.5 and 25°C Irpex lacteus
39.3
-
L-sorbose at pH 6.5 and 25°C Irpex lacteus
46.4
-
maltose at pH 6.5 and 25°C Irpex lacteus
51.1
-
D-glucono-1,5-lactone at pH 6.5 and 25°C Irpex lacteus
290
-
cellobiose at pH 6.5 and 25°C Irpex lacteus
370
-
D-mannose at pH 6.5 and 25°C Irpex lacteus
727
-
D-fructose at pH 6.5 and 25°C Irpex lacteus
902
-
sucrose at pH 6.5 and 25°C Irpex lacteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
-
Irpex lacteus
266000
-
gel filtration Irpex lacteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + O2 Irpex lacteus preferred substrate 2-dehydro-D-glucose + H2O2
-
?
D-glucose + O2 Irpex lacteus dft-1 preferred substrate 2-dehydro-D-glucose + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Irpex lacteus
-
-
-
Irpex lacteus dft-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl Sepharose 6 column chromatography, DEAE-Sepharose column chromatography, and Sephacryl S-300 gel filtration Irpex lacteus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Irpex lacteus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.31
-
unpurified enzyme, at pH 6.5 and 25°C Irpex lacteus
8.64
-
after 27.9fold purification, at pH 6.5 and 25°C Irpex lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + O2 17.8% activity compared to D-glucose Irpex lacteus 2-dehydrocellobiose + H2O2
-
?
D-fructose + O2 12.4% activity compared to D-glucose Irpex lacteus 2-dehydro-D-fructose + H2O2
-
?
D-galactose + O2 38.8% activity compared to D-glucose Irpex lacteus 2-dehydro-D-galactose + H2O2
-
?
D-galactose + O2 38.8% activity compared to D-glucose Irpex lacteus dft-1 2-dehydro-D-galactose + H2O2
-
?
D-glucono-1,5-lactone + O2 107% activity compared to D-glucose Irpex lacteus 2-dehydro-D-glucono-1,5-lactone + H2O2
-
?
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
Irpex lacteus 2-dehydro-D-glucose + ?
-
?
D-glucose + O2 preferred substrate Irpex lacteus 2-dehydro-D-glucose + H2O2
-
?
D-glucose + O2 100% activity Irpex lacteus 2-dehydro-D-glucose + H2O2
-
?
D-glucose + O2 preferred substrate Irpex lacteus dft-1 2-dehydro-D-glucose + H2O2
-
?
D-glucose + O2 100% activity Irpex lacteus dft-1 2-dehydro-D-glucose + H2O2
-
?
D-mannose + O2 18.4% activity compared to D-glucose Irpex lacteus 2-dehydro-D-mannose + H2O2
-
?
D-xylose + O2 75.1% activity compared to D-glucose Irpex lacteus 2-dehydro-D-xylose + H2O2
-
?
L-sorbose + O2 122% activity compared to D-glucose Irpex lacteus 5-dehydro-D-fructose + H2O2
-
?
L-sorbose + O2 122% activity compared to D-glucose Irpex lacteus dft-1 5-dehydro-D-fructose + H2O2
-
?
lactose + O2 0.71% activity compared to D-glucose Irpex lacteus 2-dehydrolactose + H2O2
-
?
maltose + O2 92.2% activity compared to D-glucose Irpex lacteus 2-dehydro-D-maltose + H2O2
-
?
additional information no activity with D-arabinose Irpex lacteus ?
-
?
additional information no activity with D-arabinose Irpex lacteus dft-1 ?
-
?
sucrose + O2 82.8% activity compared to D-glucose Irpex lacteus ? + H2O2
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 71000, SDS-PAGE Irpex lacteus

Synonyms

Synonyms Comment Organism
C-2 specific pyranose-2-oxidase
-
Irpex lacteus
P2O
-
Irpex lacteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Irpex lacteus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65 the enzyme shows less than half of its maximum activity at 30°C and 65°C, respectively Irpex lacteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 the enzyme remains stable up to 60°C. At 70°C, more than half of its initial activity is retained after 30 min of incubation in phosphate buffer, pH 6.5. At 80°C, it is totally inactivated within 30 min Irpex lacteus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.19
-
D-fructose at pH 6.5 and 25°C Irpex lacteus
6.01
-
cellobiose at pH 6.5 and 25°C Irpex lacteus
6.2
-
D-mannose at pH 6.5 and 25°C Irpex lacteus
13.1
-
D-galactose at pH 6.5 and 25°C Irpex lacteus
25.4
-
D-xylose at pH 6.5 and 25°C Irpex lacteus
27.9
-
sucrose at pH 6.5 and 25°C Irpex lacteus
31.1
-
maltose at pH 6.5 and 25°C Irpex lacteus
33.8
-
D-glucose at pH 6.5 and 25°C Irpex lacteus
36.1
-
D-glucono-1,5-lactone at pH 6.5 and 25°C Irpex lacteus
41.4
-
L-sorbose at pH 6.5 and 25°C Irpex lacteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Irpex lacteus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 at pH 5.0 in citrate buffer and pH 9.0 in glycine-NaOH buffer, the enzyme displays about 60% of its maximum activity. The activity begins to decline dramatically below pH 5.0 and above pH 9.0 Irpex lacteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 almost no activity loss occurs in the pH range 5.0-11.0 on incubation for 24 h at 25°C. Nearly half and 80% of its initial activity remains at pH 3.0 and 3.5, respectively Irpex lacteus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.006
-
D-fructose at pH 6.5 and 25°C Irpex lacteus
0.017
-
D-mannose at pH 6.5 and 25°C Irpex lacteus
0.021
-
cellobiose at pH 6.5 and 25°C Irpex lacteus
0.031
-
sucrose at pH 6.5 and 25°C Irpex lacteus
0.67
-
maltose at pH 6.5 and 25°C Irpex lacteus
0.71
-
D-glucono-1,5-lactone at pH 6.5 and 25°C Irpex lacteus
0.98
-
D-xylose at pH 6.5 and 25°C Irpex lacteus
1.05
-
L-sorbose at pH 6.5 and 25°C Irpex lacteus
1.7
-
D-galactose at pH 6.5 and 25°C Irpex lacteus
45.6
-
D-glucose at pH 6.5 and 25°C Irpex lacteus