Application | Comment | Organism |
---|---|---|
biotechnology | biocatalyst for carbohydrate transformations toward higher-value products | Trametes ochracea |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star DE3 cells | Trametes ochracea |
Protein Variants | Comment | Organism |
---|---|---|
E542K | the mutant is characterized by reduced KM values for both D-glucose and D-galactose and significantly increased stability | Trametes ochracea |
H450G | the mutant shows a 3.6fold decrease in KM together with a 1.4fold increase in kcat for its substrate D-galactose and an overall improvement in the catalytic efficiency by a factor of 5 compared to the wild type enzyme | Trametes ochracea |
H450G/E542K/V546C | mutant shows catalytic efficiency for its substrate D-galactose that is increased 1.2fold compared to the wild type enzyme while kcat/KM for D-glucose is decreased to 17% of its original value | Trametes ochracea |
H450G/V546C | mutant with increased activity with D-galactose, high activity with D-glucose, and considerably increased stability for the latter variant | Trametes ochracea |
V546C | the mutant is characterized by elevated kcat values for both sugar substrates D-glucose and D-galactose, which is accompanied by elevated KM values | Trametes ochracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.521 | - |
D-glucose | mutant enzyme E542K, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
0.77 | - |
D-glucose | mutant enzyme H450G/E542K/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
0.987 | - |
D-glucose | mutant enzyme H450G, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
0.99 | - |
D-glucose | His-tagged recombinant wild type enzyme, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
2.43 | - |
D-glucose | mutant enzyme H450G/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
2.45 | - |
D-galactose | mutant enzyme H450G, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
3.06 | - |
D-glucose | mutant enzyme V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
3.87 | - |
D-galactose | mutant enzyme E542K, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
7.8 | - |
D-galactose | mutant enzyme H450G/E542K/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
8.79 | - |
D-galactose | His-tagged recombinant wild type enzyme, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
12 | - |
D-galactose | mutant enzyme H450G/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
46.2 | - |
D-galactose | mutant enzyme V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | Q7ZA32 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + O2 | His-tagged recombinant wild type enzyme strongly prefers D-glucose to D-galactose as its substrate | Trametes ochracea | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-glucose + O2 | His-tagged recombinant wild type enzyme strongly prefers D-glucose to D-galactose as its substrate | Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Trametes ochracea |
Synonyms | Comment | Organism |
---|---|---|
glucose 2-oxidase | - |
Trametes ochracea |
P2Ox | - |
Trametes ochracea |
pyranose 2-Oxidase | - |
Trametes ochracea |
pyranose:oxygen 2-oxidoreductase | - |
Trametes ochracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | the wild type enzyme shows a half life of 10 min at 60°C and of less than 1 min at 70°C | Trametes ochracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.51 | - |
D-galactose | His-tagged recombinant wild type enzyme, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
2.59 | - |
D-galactose | mutant enzyme E542K, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
2.84 | - |
D-galactose | mutant enzyme H450G/E542K/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
3.51 | - |
D-galactose | mutant enzyme H450G, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
5.92 | - |
D-galactose | mutant enzyme H450G/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
6.57 | - |
D-galactose | mutant enzyme V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
6.81 | - |
D-glucose | mutant enzyme H450G/E542K/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
12.5 | - |
D-glucose | mutant enzyme H450G, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
16.8 | - |
D-glucose | mutant enzyme H450G/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
35.9 | - |
D-glucose | mutant enzyme E542K, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
48.1 | - |
D-glucose | His-tagged recombinant wild type enzyme, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
88.6 | - |
D-glucose | mutant enzyme V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trametes ochracea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.142 | - |
D-galactose | mutant enzyme V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
0.286 | - |
D-galactose | His-tagged recombinant wild type enzyme, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
0.364 | - |
D-galactose | mutant enzyme H450G/E542K/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
0.493 | - |
D-galactose | mutant enzyme H450G/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
0.669 | - |
D-galactose | mutant enzyme E542K, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
1.43 | - |
D-galactose | mutant enzyme H450G, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
8.83 | - |
D-glucose | mutant enzyme H450G/E542K/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
12.7 | - |
D-glucose | mutant enzyme H450G, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
13.5 | - |
D-glucose | mutant enzyme H450G/V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
29 | - |
D-glucose | mutant enzyme V546C, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
51.2 | - |
D-glucose | His-tagged recombinant wild type enzyme, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea | |
68.9 | - |
D-glucose | mutant enzyme E542K, in 50 mM phosphate buffer (pH 6.5) at 60°C | Trametes ochracea |