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Literature summary for 1.1.3.10 extracted from

  • Danneel, H.J.; Rossner, E.; Zeeck, A.; Giffhorn, F.
    Purification and characterization of a pyranose oxidase from the basidiomycete Peniophora gigantea and chemical analyses of its reaction products (1993), Eur. J. Biochem., 214, 795-802.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline no significant effect Phlebiopsis gigantea
2-mercaptoethanol 10% inhibition at 1 mM, 95% inhibition at 10 mM Phlebiopsis gigantea
Ag+ completely inhibits at 1 mM Phlebiopsis gigantea
alpha,alpha'-dipyridyl no significant effect Phlebiopsis gigantea
ascorbic acid 10% inhibition at 1 mM, 95% inhibition at 10 mM Phlebiopsis gigantea
Dithionite 10% inhibition at 1 mM, 95% inhibition at 10 mM Phlebiopsis gigantea
dithiothreitol 10% inhibition at 1 mM, 95% inhibition at 10 mM Phlebiopsis gigantea
Fe2+ completely inhibits at 1 mM Phlebiopsis gigantea
N-bromosuccinimide completely inhibitory Phlebiopsis gigantea
p-chloromercuribenzoate completely inhibits at 1 mM Phlebiopsis gigantea
reduced glutathione 10% inhibition at 1 mM, 95% inhibition at 10 mM Phlebiopsis gigantea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.65
-
oxygen in the presence of glucose Phlebiopsis gigantea
1.1
-
D-glucose
-
Phlebiopsis gigantea
29.4
-
D-xylose
-
Phlebiopsis gigantea
50
-
L-sorbose
-
Phlebiopsis gigantea

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slightly stimulates at 1 mM Phlebiopsis gigantea
Co2+ slightly stimulates at 1 mM Phlebiopsis gigantea
Mg2+ slightly stimulates at 1 mM Phlebiopsis gigantea
Zn2+ slightly stimulates at 1 mM Phlebiopsis gigantea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
4 * 76000, SDS-PAGE Phlebiopsis gigantea
322800
-
determined on the basis of its Stokes radius and sedimentation coefficient, dynamic light-scattering experiments, gradient-gel electrophoresis and cross-linking studies Phlebiopsis gigantea

Organism

Organism UniProt Comment Textmining
Phlebiopsis gigantea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using fractionated ammonium sulfate precipitation, chromatography on DEAE-Sephacel, Sephacryl S 300, S Sepharose and Q Sepharose Phlebiopsis gigantea

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.4
-
-
Phlebiopsis gigantea

Storage Stability

Storage Stability Organism
repeated freezing at -20°C and thawing results in a rapid and irreversible loss of activity Phlebiopsis gigantea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-melibiose + O2 1.9% relative activity to D-glucose Phlebiopsis gigantea ? + H2O2
-
?
D-fucose + O2 2.9% relative activity to D-glucose Phlebiopsis gigantea ?
-
?
D-galactose + O2 7.7% relative activity to D-glucose Phlebiopsis gigantea 2-dehydro-D-galactose + H2O2
-
?
D-gluconolactone + O2 1.5% relative activity to D-glucose Phlebiopsis gigantea ? + H2O2
-
?
D-glucose + O2 activation energy for the conversion of D-glucose is 34.6 kJ/mol Phlebiopsis gigantea D-glucosone + H2O2
-
?
D-glucose + O2 D-glucose is the preferred substrate Phlebiopsis gigantea D-glucosone + H2O2
-
?
D-xylose + O2 50% relative activity to D-glucose Phlebiopsis gigantea D-xylosone + H2O2
-
?
L-sorbose + O2 109% relative activity to D-glucose Phlebiopsis gigantea 5-dehydro-D-fructose + H2O2
-
?
maltose + O2 8.2% relative activity to D-glucose Phlebiopsis gigantea ? + H2O2
-
?
methyl alpha-D-glucoside + O2 1.9% relative activity to D-glucose Phlebiopsis gigantea 2-dehydro-alpha-D-methylglucoside + H2O2
-
?
methyl beta-D-glucoside + O2 9.6% relative activity to D-glucose Phlebiopsis gigantea 2-dehydro-beta-D-methylglucoside + H2O2
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 76000, SDS-PAGE Phlebiopsis gigantea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
Phlebiopsis gigantea

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound Phlebiopsis gigantea
FAD 3.6 molecules FAD per molecule enzyme Phlebiopsis gigantea