Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.2.B5 extracted from

  • Takeda, K.; Matsumura, H.; Ishida, T.; Samejima, M.; Ohno, H.; Yoshida, M.; Igarashi, K.; Nakamura, N.
    Characterization of a novel PQQ-dependent quinohemoprotein pyranose dehydrogenase from Coprinopsis cinerea classified into auxiliary activities family 12 in carbohydrate-active enzymes (2015), PLoS ONE, 10, e0115722.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Coprinopsis cinerea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.9
-
D-glucosone at pH 8.5 and 30°C Coprinopsis cinerea
23.1
-
D-talose at pH 8.5 and 30°C Coprinopsis cinerea
24.8
-
L-fucose at pH 8.5 and 30°C Coprinopsis cinerea
30.3
-
D-arabinose at pH 8.5 and 30°C Coprinopsis cinerea
49.7
-
L-galactose at pH 8.5 and 30°C Coprinopsis cinerea
66.8
-
D-Lyxose at pH 8.5 and 30°C Coprinopsis cinerea
84.7
-
L-gulose at pH 8.5 and 30°C Coprinopsis cinerea
236
-
L-Xylose at pH 8.5 and 30°C Coprinopsis cinerea
363
-
L-Glucose at pH 8.5 and 30°C Coprinopsis cinerea

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM Ca2+ is used in assay conditions Coprinopsis cinerea

Organism

Organism UniProt Comment Textmining
Coprinopsis cinerea
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinose + 2,6-dichlorophenolindophenol 47.5% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
?
D-glucosone + ferricytochrome c 100% activity Coprinopsis cinerea ? + ferrocytochrome c
-
?
D-lyxose + 2,6-dichlorophenolindophenol 13.4% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
?
D-talose + ferricytochrome c 24.8% activity compared to D-glucosone Coprinopsis cinerea ? + ferrocytochrome c
-
?
L-fucose + 2,6-dichlorophenolindophenol 77.7% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
?
L-galactose + ferricytochrome c 65.4% activity compared to D-glucosone Coprinopsis cinerea ? + ferrocytochrome c
-
?
L-glucose + ferricytochrome c 9.2% activity compared to D-glucosone Coprinopsis cinerea ? + ferrocytochrome c
-
?
L-gulose + 2,6-dichlorophenolindophenol 52.1% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
?
L-lyxose + 2,6-dichlorophenolindophenol 4.0% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
?
L-mannose + 2,6-dichlorophenolindophenol 3.6% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
?
L-rhamnose + 2,6-dichlorophenolindophenol 0.7% activity compared to D-glucosone Coprinopsis cinerea ? + reduced 2,6-dichlorophenolindophenol
-
?
L-xylose + ferricytochrome c 11.9% activity compared to D-glucosone Coprinopsis cinerea ? + ferrocytochrome c
-
?
additional information the enzyme prefers monosaccharides with equatorial C-2, C-3 hydroxyl groups and an axial C-4 hydroxyl group in the 1C4 chair conformation Coprinopsis cinerea ?
-
?

Synonyms

Synonyms Comment Organism
PDH
-
Coprinopsis cinerea
PQQ-dependent quinohemoprotein pyranose dehydrogenase
-
Coprinopsis cinerea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.9
-
D-Lyxose at pH 8.5 and 30°C Coprinopsis cinerea
17.8
-
D-talose at pH 8.5 and 30°C Coprinopsis cinerea
22.4
-
L-Xylose at pH 8.5 and 30°C Coprinopsis cinerea
24.9
-
L-Glucose at pH 8.5 and 30°C Coprinopsis cinerea
35.5
-
D-arabinose at pH 8.5 and 30°C Coprinopsis cinerea
53.3
-
L-gulose at pH 8.5 and 30°C Coprinopsis cinerea
56.1
-
L-galactose at pH 8.5 and 30°C Coprinopsis cinerea
56.4
-
L-fucose at pH 8.5 and 30°C Coprinopsis cinerea
74.1
-
D-glucosone at pH 8.5 and 30°C Coprinopsis cinerea

Cofactor

Cofactor Comment Organism Structure
heme b
-
Coprinopsis cinerea
pyrroloquinoline quinone
-
Coprinopsis cinerea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.07
-
L-Glucose at pH 8.5 and 30°C Coprinopsis cinerea
0.09
-
L-Xylose at pH 8.5 and 30°C Coprinopsis cinerea
0.19
-
D-Lyxose at pH 8.5 and 30°C Coprinopsis cinerea
0.63
-
L-gulose at pH 8.5 and 30°C Coprinopsis cinerea
0.77
-
D-talose at pH 8.5 and 30°C Coprinopsis cinerea
1.13
-
L-galactose at pH 8.5 and 30°C Coprinopsis cinerea
1.17
-
D-arabinose at pH 8.5 and 30°C Coprinopsis cinerea
2.27
-
L-fucose at pH 8.5 and 30°C Coprinopsis cinerea
9.37
-
D-glucosone at pH 8.5 and 30°C Coprinopsis cinerea