Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Coprinopsis cinerea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.9 | - |
D-glucosone | at pH 8.5 and 30°C | Coprinopsis cinerea | |
23.1 | - |
D-talose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
24.8 | - |
L-fucose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
30.3 | - |
D-arabinose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
49.7 | - |
L-galactose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
66.8 | - |
D-Lyxose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
84.7 | - |
L-gulose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
236 | - |
L-Xylose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
363 | - |
L-Glucose | at pH 8.5 and 30°C | Coprinopsis cinerea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM Ca2+ is used in assay conditions | Coprinopsis cinerea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis cinerea | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + 2,6-dichlorophenolindophenol | 47.5% activity compared to D-glucosone | Coprinopsis cinerea | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucosone + ferricytochrome c | 100% activity | Coprinopsis cinerea | ? + ferrocytochrome c | - |
? | |
D-lyxose + 2,6-dichlorophenolindophenol | 13.4% activity compared to D-glucosone | Coprinopsis cinerea | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-talose + ferricytochrome c | 24.8% activity compared to D-glucosone | Coprinopsis cinerea | ? + ferrocytochrome c | - |
? | |
L-fucose + 2,6-dichlorophenolindophenol | 77.7% activity compared to D-glucosone | Coprinopsis cinerea | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-galactose + ferricytochrome c | 65.4% activity compared to D-glucosone | Coprinopsis cinerea | ? + ferrocytochrome c | - |
? | |
L-glucose + ferricytochrome c | 9.2% activity compared to D-glucosone | Coprinopsis cinerea | ? + ferrocytochrome c | - |
? | |
L-gulose + 2,6-dichlorophenolindophenol | 52.1% activity compared to D-glucosone | Coprinopsis cinerea | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-lyxose + 2,6-dichlorophenolindophenol | 4.0% activity compared to D-glucosone | Coprinopsis cinerea | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-mannose + 2,6-dichlorophenolindophenol | 3.6% activity compared to D-glucosone | Coprinopsis cinerea | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-rhamnose + 2,6-dichlorophenolindophenol | 0.7% activity compared to D-glucosone | Coprinopsis cinerea | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-xylose + ferricytochrome c | 11.9% activity compared to D-glucosone | Coprinopsis cinerea | ? + ferrocytochrome c | - |
? | |
additional information | the enzyme prefers monosaccharides with equatorial C-2, C-3 hydroxyl groups and an axial C-4 hydroxyl group in the 1C4 chair conformation | Coprinopsis cinerea | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Coprinopsis cinerea |
PQQ-dependent quinohemoprotein pyranose dehydrogenase | - |
Coprinopsis cinerea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.9 | - |
D-Lyxose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
17.8 | - |
D-talose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
22.4 | - |
L-Xylose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
24.9 | - |
L-Glucose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
35.5 | - |
D-arabinose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
53.3 | - |
L-gulose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
56.1 | - |
L-galactose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
56.4 | - |
L-fucose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
74.1 | - |
D-glucosone | at pH 8.5 and 30°C | Coprinopsis cinerea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme b | - |
Coprinopsis cinerea | |
pyrroloquinoline quinone | - |
Coprinopsis cinerea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
L-Glucose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
0.09 | - |
L-Xylose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
0.19 | - |
D-Lyxose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
0.63 | - |
L-gulose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
0.77 | - |
D-talose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
1.13 | - |
L-galactose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
1.17 | - |
D-arabinose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
2.27 | - |
L-fucose | at pH 8.5 and 30°C | Coprinopsis cinerea | |
9.37 | - |
D-glucosone | at pH 8.5 and 30°C | Coprinopsis cinerea |