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Literature summary for 1.1.2.B2 extracted from

  • Davidson, V.L.; Wu, J.; Miller, B.; Jones, L.H.
    Factors affecting the stability of methanol dehydrogenase from Paracoccus denitrificans (1992), FEMS Microbiol. Lett., 94, 53-58.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA decreases enzyme thermal stability Paracoccus denitrificans
KCN
-
Paracoccus denitrificans

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Paracoccus denitrificans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ increases enzyme thermal stability, Ca2+ has a structural role Paracoccus denitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
x * 67000 + x * 12000, SDS-PAGE Paracoccus denitrificans
67000
-
x * 67000 + x * 12000, SDS-PAGE Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
strain ATCC 13543
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 6.8fold from periplasm to homogeneity by ultrafiltration, dialysis, anion exchange chromatography, and gel filtration Paracoccus denitrificans

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:methanol-grown cell
-
Paracoccus denitrificans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4
-
purified enzyme Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + nitroblue tetrazolium
-
Paracoccus denitrificans formaldehyde + reduced nitroblue tetrazolium
-
?

Subunits

Subunits Comment Organism
? x * 67000 + x * 12000, SDS-PAGE Paracoccus denitrificans

Synonyms

Synonyms Comment Organism
methanol dehydrogenase
-
Paracoccus denitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Paracoccus denitrificans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 60 10 min, the purified enzyme is quite stable within this range, significant loss of activity occurs at higher temperatures, Ca2+ enhances the thermal stability, while EDTA reduces it Paracoccus denitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
and above Paracoccus denitrificans

pH Range

pH Minimum pH Maximum Comment Organism
4.5 11.5
-
Paracoccus denitrificans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9 30°C, 20 h, stable below pH 9, rapid loss of activity above pH 9 Paracoccus denitrificans

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone non-covalently bound Paracoccus denitrificans