Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | decreases enzyme thermal stability | Paracoccus denitrificans | |
KCN | - |
Paracoccus denitrificans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Paracoccus denitrificans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | increases enzyme thermal stability, Ca2+ has a structural role | Paracoccus denitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
x * 67000 + x * 12000, SDS-PAGE | Paracoccus denitrificans |
67000 | - |
x * 67000 + x * 12000, SDS-PAGE | Paracoccus denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | - |
strain ATCC 13543 | - |
Purification (Comment) | Organism |
---|---|
native enzyme 6.8fold from periplasm to homogeneity by ultrafiltration, dialysis, anion exchange chromatography, and gel filtration | Paracoccus denitrificans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:methanol-grown cell | - |
Paracoccus denitrificans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.4 | - |
purified enzyme | Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + nitroblue tetrazolium | - |
Paracoccus denitrificans | formaldehyde + reduced nitroblue tetrazolium | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000 + x * 12000, SDS-PAGE | Paracoccus denitrificans |
Synonyms | Comment | Organism |
---|---|---|
methanol dehydrogenase | - |
Paracoccus denitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Paracoccus denitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | 10 min, the purified enzyme is quite stable within this range, significant loss of activity occurs at higher temperatures, Ca2+ enhances the thermal stability, while EDTA reduces it | Paracoccus denitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
and above | Paracoccus denitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 11.5 | - |
Paracoccus denitrificans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9 | 30°C, 20 h, stable below pH 9, rapid loss of activity above pH 9 | Paracoccus denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | non-covalently bound | Paracoccus denitrificans |