Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.2.3 extracted from

  • Eprintsev, A.; Larchenkov, V.; Komarova, N.; Kovaleva, E.; Mitkevich, A.; Falaleeva, M.; Kompantseva, E.
    Purification and investigation of physicochemical and regulatory properties of homogeneous L-lactate cytochrom c oxidoreductase obtained from the nonsulfur purple bacterium Rhodovulum steppense (2018), Appl. Biochem. Microbiol., 54, 370-374 .
No PubMed abstract available
Search for more literature for 1.1.2.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 (S)-lactate pH and temperature not specified in the publication Rhodovulum steppense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
-
 Rhodovulum steppense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Rhodovulum steppense
-
 pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
(S)-lactate + 2 ferricytochrome c Rhodovulum steppense A-20s
-
 pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodovulum steppense
-
-
-
Rhodovulum steppense A-20s
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodovulum steppense

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.75
-
 pH and temperature not specified in the publication Rhodovulum steppense

Storage Stability

Storage Stability Organism
-20°C, 1 week, the enzyme preparation retains 90% of its activity Rhodovulum steppense
-20°C, 6 months, the enzyme preparation retains 4% of its activity Rhodovulum steppense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 2 ferricytochrome c
-
 Rhodovulum steppense pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
(S)-lactate + 2 ferricytochrome c
-
 Rhodovulum steppense A-20s pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 68000, Na-DDS electrophoresis Rhodovulum steppense

Synonyms

Synonyms Comment Organism
L-lactate:cytochrom c oxidoreductase
-
 Rhodovulum steppense
L-LCO
-
 Rhodovulum steppense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
31
-
-
 Rhodovulum steppense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Rhodovulum steppense

pH Range

pH Minimum pH Maximum Comment Organism
7.6 8.5 pH 7.6: about 50% of maximal activity, pH 8.5: about 60% of maximal activity Rhodovulum steppense