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Literature summary for 1.1.2.3 extracted from

  • Gondry, M.; Duboist, J.; Terrier, M.; Lederer, F.
    The catalytic role of tyrosine 254 in flavocytochrome b2 (L-lactate dehydrogenase from baker's yeast). Comparison between the Y254F and Y254L mutant proteins (2001), Eur. J. Biochem., 268, 4918-4927.
    View publication on PubMed
Search for more literature for 1.1.2.3

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli MM294 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
Y254F only traces of activity Saccharomyces cerevisiae
Y254L turnover reduced to less than 10% Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
D-lactate
-
 Saccharomyces cerevisiae
L-Mandelate competitive Saccharomyces cerevisiae
oxalate
-
 Saccharomyces cerevisiae
pyruvate competitive at low concentrations, non-competitve at high concentrations Saccharomyces cerevisiae
sulfite competitive Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial intermembrane space
-
 Saccharomyces cerevisiae 5758
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound to enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 2 ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
(S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae ?
-
 ?
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae