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Literature summary for 1.1.2.3 extracted from
- X-ray structure of two complexes of the Y143F flavocytochrome b2 mutant crystallized in the presence of lactate or phenyl lactate (1995), Biochemistry, 34, 9840-9850.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y143F | - |
Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
L-lactate | Y143F mutant with cytochrome c as electron acceptor | Saccharomyces cerevisiae |  |
| 0.24 | - |
L-lactate | native enzyme with cytochrome c as electron acceptor | Saccharomyces cerevisiae | |
| 0.49 | - |
L-lactate | native enzyme mutant with ferricyanide as electron acceptor | Saccharomyces cerevisiae | |
| 2.9 | - |
L-lactate | Y143F mutant with ferricyanide as electron acceptor | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial intermembrane space | soluble | Saccharomyces cerevisiae | 5758 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | bound to enzyme | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-lactate + 2 ferricytochrome c | Saccharomyces cerevisiae | can feed electrons to respiratory chain at the level of cytochrome c | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, precipitate from 70% saturated ammonium sulfate, under nitrogen, stable for several weeks | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-lactate + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
| (S)-lactate + 2 ferricytochrome c | can feed electrons to respiratory chain at the level of cytochrome c | Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
| additional information | ferricyanide used as electron acceptor | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
L-lactate | Y143F mutant with cytochrome c as electron acceptor | Saccharomyces cerevisiae | |
| 103 | - |
L-lactate | native enzyme with cytochrome c as electron acceptor | Saccharomyces cerevisiae | |
| 400 | - |
L-lactate | native enzyme and Y143F mutant with ferricyanide as electron acceptor | Saccharomyces cerevisiae | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Saccharomyces cerevisiae | |
| heme | - |
Saccharomyces cerevisiae | |
| additional information | ferricyanide used as electron acceptor | Saccharomyces cerevisiae | |
| additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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