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Literature summary for 1.1.2.3 extracted from
- A standard high-yield purification of L-(+)-lactate: cytochrome c oxidoreductase (cytochrome b2) from the yeast Hansenula anomala (1978), Anal. Biochem., 88, 624-633.
General Stability
| General Stability | Organism |
|---|---|
| PMSF stabilizes, purification in absence of PMSF yields cleaved form of enzyme | Wickerhamomyces anomalus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | bound to enzyme | Wickerhamomyces anomalus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62750 | - |
4 * 62750, SDS-PAGE | Wickerhamomyces anomalus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-lactate + 2 ferricytochrome c | Wickerhamomyces anomalus | - |
pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Wickerhamomyces anomalus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Wickerhamomyces anomalus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 895 | - |
purified enzyme | Wickerhamomyces anomalus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, saturated ammonium sulfate, under nitrogen, protected from light, stabe | Wickerhamomyces anomalus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-lactate + 2 ferricytochrome c | - |
Wickerhamomyces anomalus | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
| additional information | electron acceptors other than ferricytochrome c used | Wickerhamomyces anomalus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 62750, SDS-PAGE | Wickerhamomyces anomalus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | electron acceptors other than ferricytochrome c used | Wickerhamomyces anomalus |
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Release 2023.1 (January 2023)
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