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Literature summary for 1.1.2.2 extracted from
- Mannitol (cytochrome) dehydrogenase (1966), Methods Enzymol., 9, 147-149.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 17 | - |
D-arabitol | - |
Gluconobacter oxydans |  |
| 20 | - |
D-sorbitol | - |
Gluconobacter oxydans | |
| 31 | - |
D-mannitol | - |
Gluconobacter oxydans | |
| 44 | - |
ribitol | - |
Gluconobacter oxydans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | restores activity after inactivation with EDTA and storage | Gluconobacter oxydans | |
| Mg2+ | restores activity after inactivation with EDTA and storage | Gluconobacter oxydans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol + ferricytochrome c | Gluconobacter oxydans | - |
D-fructose + ferrocytochrome c | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, stable for several weeks, lost activity can be restored by adding Ca2+ or Mg2+ | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyclic D-erythro polyols + ferricytochrome c | activity found with erythritiol, ribitol, D-arabinitol, D-sorbitol and others | Gluconobacter oxydans | acyclic D-erythro ketoses + ferrocytochrome c | - |
? | |
| D-arabitol + ferricytochrome c | - |
Gluconobacter oxydans | ? | - |
r | |
| D-mannitol + ferricytochrome c | - |
Gluconobacter oxydans | D-fructose + ferrocytochrome c | - |
? | |
| D-mannitol + ferricytochrome c | other hydrogen acceptors: phenol blue, ferricyanide, phenolindo-2,6-dichlorophenol, o-chlorophenolindo-2,6-dichlorophenol | Gluconobacter oxydans | D-fructose + ferrocytochrome c | - |
? | |
| D-sorbitol + ferricytochrome c | - |
Gluconobacter oxydans | D-fructose + ferrocytochrome c | - |
? | |
| ribitol + ferricytochrome c | - |
Gluconobacter oxydans | D-ribulose + ferrocytochrome c | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | other electron acceptors: phenol blue, ferricyanide, phenolindo-2,6-dichlorophenol, o-chlorophenolindo-2,6-dichlorophenol | Gluconobacter oxydans |
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Release 2023.1 (January 2023)
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