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Literature summary for 1.1.2.10 extracted from

  • Versantvoort, W.; Pol, A.; Daumann, L.J.; Larrabee, J.A.; Strayer, A.H.; Jetten, M.S.M.; van Niftrik, L.; Reimann, J.; Op den Camp, H.J.M.
    Characterization of a novel cytochrome cGJ as the electron acceptor of XoxF-MDH in the thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV (2019), Biochim. Biophys. Acta, 1867, 595-603 .
    View publication on PubMed
Search for more literature for 1.1.2.10

Inhibitors

Inhibitors Comment Organism Structure
NaCl about 60% inhibition at 300 mM Methylacidiphilum fumariolicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + 2 cytochrome cGJ Methylacidiphilum fumariolicum
-
 formaldehyde + 2 reduced cytochrome cGJ
-
 r
methanol + 2 cytochrome cGJ Methylacidiphilum fumariolicum SolV
-
 formaldehyde + 2 reduced cytochrome cGJ
-
 r

Organism

Organism UniProt Comment Textmining
Methylacidiphilum fumariolicum
-
-
-
Methylacidiphilum fumariolicum SolV
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + 2 cytochrome cGJ
-
 Methylacidiphilum fumariolicum formaldehyde + 2 reduced cytochrome cGJ
-
 r
methanol + 2 cytochrome cGJ electrochemical redox reaction Methylacidiphilum fumariolicum formaldehyde + 2 reduced cytochrome cGJ
-
 r
methanol + 2 cytochrome cGJ
-
 Methylacidiphilum fumariolicum SolV formaldehyde + 2 reduced cytochrome cGJ
-
 r
methanol + 2 cytochrome cGJ electrochemical redox reaction Methylacidiphilum fumariolicum SolV formaldehyde + 2 reduced cytochrome cGJ
-
 r

Subunits

Subunits Comment Organism
? x * 29783, CytcGJ with heme c, mass spectrometry, x * 25000, CytcGJ with heme c, SDS-PAGE Methylacidiphilum fumariolicum

Synonyms

Synonyms Comment Organism
lanthanide-dependent XoxF-type MDH
-
 Methylacidiphilum fumariolicum
lanthanide-dependent XoxF-type methanol dehydrogenase
-
 Methylacidiphilum fumariolicum
XoxF-MDH
-
 Methylacidiphilum fumariolicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at Methylacidiphilum fumariolicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Methylacidiphilum fumariolicum

Cofactor

Cofactor Comment Organism Structure
cytochrome cGJ encoded by gene Mfumv2_1185, the cytochrome cL homologue from the strictly lanthanide-dependent, thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV is a fusion of a XoxG cytochrome and a periplasmic binding protein XoxJ. XoxGJ functions as the direct electron acceptor of its corresponding XoxF-type MDH and can sustain methanol turnover, when a secondary cytochrome is present as final electron acceptor. SolV cytochrome cGJ (XoxGJ) further displays a unique, red-shifted absorbance spectrum, with a Soret and Q bands at 440, 553 and 595 nm in the reduced state, respectively. VTVH-MCD spectroscopy reveals the presence of a low spin iron heme and the data further shows that the heme group exhibits minimal ruffling. The midpoint potential Em,pH7 of +240 mV is similar to other cytochrome cL type proteins but the midpoint potential of cytochrome cGJ is not influenced by lowering the pH. Covalently attached heme c moiety, the heme modification of SolV cytochrome cGJ, might stabilize it at lower pH and prevent the increase in midpoint potential Methylacidiphilum fumariolicum