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Literature summary for 1.1.2.10 extracted from
- Contrasting in vitro and in vivo methanol oxidation activities of lanthanide-dependent alcohol dehydrogenases XoxF1 and ExaF from Methylobacterium extorquens AM1 (2019), Sci. Rep., 9, 4248 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
methanol | XoxF1, in the absence of lanthanides, pH and temperature not specified in the publication | Methylorubrum extorquens |  |
| 0.014 | - |
ethanol | XoxF1, in the absence of lanthanides, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 0.029 | - |
methanol | XoxF1, in the presence of Nd3+, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 0.044 | - |
methanol | XoxF1, in the presence of La3+, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 0.065 | - |
formaldehyde | XoxF1, in the absence of lanthanides, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 0.067 | - |
ethanol | XoxF1, in the presence of La3+, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 0.096 | - |
formaldehyde | XoxF1, in the presence of La3+, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 0.133 | - |
formaldehyde | XoxF1, in the presence of Nd3+, pH and temperature not specified in the publication | Methylorubrum extorquens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| La3+ | dependent on. XoxF1 contains La3+ in 1:1 molar ratio of metal to protomer | Methylorubrum extorquens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylorubrum extorquens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + phenazine methosulfate | best substrate for ExaF | Methylorubrum extorquens | acetaldehyde + reduced phenazine methosulfate | - |
? | |
| methanol + phenazine methosulfate | best substrate for XoxF1 | Methylorubrum extorquens | formaldehyde + reduced phenazine methosulfate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exaF | - |
Methylorubrum extorquens |
| lanthanide-dependent alcohol dehydrogenase | - |
Methylorubrum extorquens |
| Ln-dependent EtDH | - |
Methylorubrum extorquens |
| Ln-dependent ethanol dehydrogenase | - |
Methylorubrum extorquens |
| Ln-dependent MetDH | - |
Methylorubrum extorquens |
| Ln-dependent methanol dehydrogenase | - |
Methylorubrum extorquens |
| xoxF1 | - |
Methylorubrum extorquens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | - |
Methylorubrum extorquens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | - |
methanol | XoxF1, in the absence of lanthanides, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 4 | - |
ethanol | XoxF1, in the absence of lanthanides, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 5 | - |
formaldehyde | XoxF1, in the absence of lanthanides, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 36 | - |
formaldehyde | XoxF1, in the presence of Nd3+, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 109 | - |
formaldehyde | XoxF1, in the presence of La3+, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 194 | - |
ethanol | XoxF1, in the presence of La3+, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 210 | - |
methanol | XoxF1, in the presence of Nd3+, pH and temperature not specified in the publication | Methylorubrum extorquens | |
| 272 | - |
methanol | XoxF1, in the presence of La3+, pH and temperature not specified in the publication | Methylorubrum extorquens | |
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