Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonia | ammonia reveals a slight inhibitory effect on subtype XoxF4-2 | Methylotenera mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
1-Hexanol | subtype XoxF4-1, at pH 9.0 and 26°C | Methylotenera mobilis | |
0.04 | - |
1-Hexanol | at pH 9.0 and 26°C | Methylomonas sp. LW13 | |
0.042 | - |
methanol | subtype XoxF4-2, at pH 9.0 and 26°C | Methylotenera mobilis | |
0.055 | - |
methanol | subtype XoxF4-1, at pH 9.0 and 26°C | Methylotenera mobilis | |
0.059 | - |
ethanol | subtype XoxF4-1, at pH 9.0 and 26°C | Methylotenera mobilis | |
0.061 | - |
1-propanol | subtype XoxF4-1, at pH 9.0 and 26°C | Methylotenera mobilis | |
0.085 | - |
ethanol | at pH 9.0 and 26°C | Methylomonas sp. LW13 | |
0.096 | - |
1-butanol | at pH 9.0 and 26°C | Methylomonas sp. LW13 | |
0.133 | - |
1-propanol | at pH 9.0 and 26°C | Methylomonas sp. LW13 | |
0.188 | - |
1-butanol | subtype XoxF4-1, at pH 9.0 and 26°C | Methylotenera mobilis | |
0.357 | - |
1-Hexanol | subtype XoxF4-2, at pH 9.0 and 26°C | Methylotenera mobilis | |
0.432 | - |
formaldehyde | subtype XoxF4-1, at pH 9.0 and 26°C | Methylotenera mobilis | |
0.464 | - |
formaldehyde | at pH 9.0 and 26°C | Methylomonas sp. LW13 | |
0.47 | - |
1-butanol | subtype XoxF4-2, at pH 9.0 and 26°C | Methylotenera mobilis | |
0.515 | - |
ethanol | subtype XoxF4-2, at pH 9.0 and 26°C | Methylotenera mobilis | |
0.815 | - |
formaldehyde | subtype XoxF4-2, at pH 9.0 and 26°C | Methylotenera mobilis | |
0.873 | - |
1-propanol | subtype XoxF4-2, at pH 9.0 and 26°C | Methylotenera mobilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ce3+ | activates | Methylotenera mobilis | |
Ce3+ | activates | Methylomonas sp. LW13 | |
Gd3+ | activates | Methylotenera mobilis | |
La3+ | activates | Methylotenera mobilis | |
La3+ | activates | Methylomonas sp. LW13 | |
additional information | subtype XoxF4-1 can use lighter lanthanides up to the atomic number of 64 (La3+ through Gd3+) while subtype XoxF4-2 can only use lanthanides up to the atomic number of 62 (La3+ through Sm3+) | Methylotenera mobilis | |
additional information | the enzyme has a requirement for ammonia | Methylomonas sp. LW13 | |
Nd3+ | activates | Methylotenera mobilis | |
Nd3+ | activates | Methylomonas sp. LW13 | |
Sm3+ | activates | Methylotenera mobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
127900 | - |
calculated from amino acid sequence | Methylomonas sp. LW13 |
129400 | - |
subtype XoxF4-1, calculated from amino acid sequence | Methylotenera mobilis |
131700 | - |
subtype XoxF4-2, calculated from amino acid sequence | Methylotenera mobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylomonas sp. LW13 | - |
- |
- |
Methylotenera mobilis | - |
- |
- |
Methylotenera mobilis JLW8 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Methylotenera mobilis |
Ni-NTA agarose column chromatography | Methylomonas sp. LW13 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:methanol-grown cell | - |
Methylotenera mobilis | - |
culture condition:methanol-grown cell | - |
Methylomonas sp. LW13 | - |
Storage Stability | Organism |
---|---|
0-25°C, the enzyme loses approximately 50% of its activity within 24 h whether maintained, on ice, in pH 8.0, pH 8.5, or pH 9.0 Tris buffer supplemented with dithiothreitol and glycerol | Methylotenera mobilis |
0-25°C, the enzyme loses approximately 50% of its activity within 24 h whether maintained, on ice, in pH 8.0, pH 8.5, or pH 9.0 Tris buffer supplemented with dithiothreitol and glycerol | Methylomonas sp. LW13 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + phenazine methosulfate | more than 95% activity compared to methanol | Methylotenera mobilis | butyraldehyde + reduced phenazine methosulfate | - |
r | |
1-butanol + phenazine methosulfate | more than 95% activity compared to methanol | Methylomonas sp. LW13 | butyraldehyde + reduced phenazine methosulfate | - |
r | |
1-hexanol + phenazine methosulfate | more than 95% activity compared to methanol | Methylotenera mobilis | hexaldehyde + reduced phenazine methosulfate | - |
r | |
1-hexanol + phenazine methosulfate | more than 95% activity compared to methanol | Methylomonas sp. LW13 | hexaldehyde + reduced phenazine methosulfate | - |
r | |
1-propanol + phenazine methosulfate | more than 95% activity compared to methanol | Methylotenera mobilis | propionaldehyde + reduced phenazine methosulfate | - |
r | |
1-propanol + phenazine methosulfate | more than 95% activity compared to methanol | Methylomonas sp. LW13 | propionaldehyde + reduced phenazine methosulfate | - |
r | |
1-propanol + phenazine methosulfate | more than 95% activity compared to methanol | Methylotenera mobilis JLW8 | propionaldehyde + reduced phenazine methosulfate | - |
r | |
2-propanol + phenazine methosulfate | 40% activity compared to methanol | Methylomonas sp. LW13 | ? | - |
r | |
2-propanol + phenazine methosulfate | subtype XoxF4-1 shows 20% activity compared to methanol, subtype XoxF4-2 shows 5% activity compared to methanol | Methylotenera mobilis | ? | - |
r | |
acetaldehyde + reduced phenazine methosulfate | 60% activity compared to methanol | Methylomonas sp. LW13 | ethanol + phenazine methosulfate | - |
r | |
acetaldehyde + reduced phenazine methosulfate | subtype XoxF4-1 shows 50% activity compared to methanol, subtype XoxF4-2 shows 15% activity compared to methanol | Methylotenera mobilis | ethanol + phenazine methosulfate | - |
r | |
acetaldehyde + reduced phenazine methosulfate | subtype XoxF4-1 shows 50% activity compared to methanol, subtype XoxF4-2 shows 15% activity compared to methanol | Methylotenera mobilis JLW8 | ethanol + phenazine methosulfate | - |
r | |
ethanol + phenazine methosulfate | more than 95% activity compared to methanol | Methylotenera mobilis | acetaldehyde + reduced phenazine methosulfate | - |
r | |
ethanol + phenazine methosulfate | more than 95% activity compared to methanol | Methylomonas sp. LW13 | acetaldehyde + reduced phenazine methosulfate | - |
r | |
ethanol + phenazine methosulfate | more than 95% activity compared to methanol | Methylotenera mobilis JLW8 | acetaldehyde + reduced phenazine methosulfate | - |
r | |
formaldehyde + reduced phenazine methosulfate | more than 95% activity compared to methanol | Methylotenera mobilis | methanol + phenazine methosulfate | - |
r | |
formaldehyde + reduced phenazine methosulfate | more than 95% activity compared to methanol | Methylomonas sp. LW13 | methanol + phenazine methosulfate | - |
r | |
methanol + phenazine methosulfate | 100% activity | Methylotenera mobilis | formaldehyde + reduced phenazine methosulfate | - |
r | |
methanol + phenazine methosulfate | 100% activity | Methylomonas sp. LW13 | formaldehyde + reduced phenazine methosulfate | - |
r | |
methanol + phenazine methosulfate | 100% activity | Methylotenera mobilis JLW8 | formaldehyde + reduced phenazine methosulfate | - |
r | |
propionaldehyde + reduced phenazine methosulfate | 60% activity compared to methanol | Methylomonas sp. LW13 | 1-propanol + phenazine methosulfate | - |
r | |
propionaldehyde + reduced phenazine methosulfate | subtype XoxF4-1 shows 50% activity compared to methanol, subtype XoxF4-2 shows 15% activity compared to methanol | Methylotenera mobilis | 1-propanol + phenazine methosulfate | - |
r | |
propionaldehyde + reduced phenazine methosulfate | subtype XoxF4-1 shows 50% activity compared to methanol, subtype XoxF4-2 shows 15% activity compared to methanol | Methylotenera mobilis JLW8 | 1-propanol + phenazine methosulfate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Methylotenera mobilis |
homodimer | - |
Methylomonas sp. LW13 |
Synonyms | Comment | Organism |
---|---|---|
lanthanide-dependent alcohol dehydrogenase | - |
Methylotenera mobilis |
lanthanide-dependent alcohol dehydrogenase | - |
Methylomonas sp. LW13 |
XoxF4-1 | subtype, dominant type in nature | Methylotenera mobilis |
XoxF4-2 | subtype | Methylotenera mobilis |
XoxF5 | - |
Methylomonas sp. LW13 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
subtype XoxF4-2 | Methylotenera mobilis |
9 | - |
- |
Methylomonas sp. LW13 |
9 | - |
subtype XoxF4-1 | Methylotenera mobilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | the enzyme shows more than 50% activity between pH 7.5 and 9.5 | Methylotenera mobilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.73 | - |
1-propanol | subtype XoxF4-2, at pH 9.0 and 26°C | Methylotenera mobilis | |
2.15 | - |
1-butanol | subtype XoxF4-2, at pH 9.0 and 26°C | Methylotenera mobilis | |
2.48 | - |
formaldehyde | subtype XoxF4-2, at pH 9.0 and 26°C | Methylotenera mobilis | |
3.97 | - |
formaldehyde | subtype XoxF4-1, at pH 9.0 and 26°C | Methylotenera mobilis | |
5.54 | - |
1-Hexanol | subtype XoxF4-2, at pH 9.0 and 26°C | Methylotenera mobilis | |
5.99 | - |
ethanol | subtype XoxF4-2, at pH 9.0 and 26°C | Methylotenera mobilis | |
16.63 | - |
1-butanol | subtype XoxF4-1, at pH 9.0 and 26°C | Methylotenera mobilis | |
27.03 | - |
ethanol | subtype XoxF4-1, at pH 9.0 and 26°C | Methylotenera mobilis | |
27.17 | - |
methanol | subtype XoxF4-1, at pH 9.0 and 26°C | Methylotenera mobilis | |
34.88 | - |
1-Hexanol | subtype XoxF4-1, at pH 9.0 and 26°C | Methylotenera mobilis | |
35.36 | - |
1-propanol | subtype XoxF4-1, at pH 9.0 and 26°C | Methylotenera mobilis | |
37.86 | - |
formaldehyde | at pH 9.0 and 26°C | Methylomonas sp. LW13 | |
42.19 | - |
methanol | subtype XoxF4-2, at pH 9.0 and 26°C | Methylotenera mobilis | |
112.54 | - |
1-propanol | at pH 9.0 and 26°C | Methylomonas sp. LW13 | |
133.9 | - |
1-butanol | at pH 9.0 and 26°C | Methylomonas sp. LW13 | |
159.15 | - |
ethanol | at pH 9.0 and 26°C | Methylomonas sp. LW13 | |
342.55 | - |
methanol | at pH 9.0 and 26°C | Methylomonas sp. LW13 | |
415.52 | - |
1-Hexanol | at pH 9.0 and 26°C | Methylomonas sp. LW13 |