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Literature summary for 1.1.2.10 extracted from
- Pyrroloquinoline quinone ethanol dehydrogenase in Methylobacterium extorquens AM1 extends lanthanide-dependent metabolism to multicarbon substrates (2016), J. Bacteriol., 198, 3109-3118 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Top10 cells | Methylorubrum extorquens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0009 | - |
ethanol | at pH 9.0 and 30°C | Methylorubrum extorquens |  |
| 0.066 | - |
formaldehyde | at pH 9.0 and 30°C | Methylorubrum extorquens | |
| 0.285 | - |
acetaldehyde | at pH 9.0 and 30°C | Methylorubrum extorquens | |
| 5.98 | - |
methanol | at pH 9.0 and 30°C | Methylorubrum extorquens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| La3+ | the enzyme preferentially binds La3+ over Ca2+ in the active site. 0.1 mM used in assay conditions. The enzyme contains 1.3 mol of La3+ per mol of protomer | Methylorubrum extorquens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 126400 | - |
SEC-MALS | Methylorubrum extorquens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylorubrum extorquens | C5AXV8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Methylorubrum extorquens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + phenazine methosulfate | highest activity | Methylorubrum extorquens | acetaldehyde + reduced phenazine methosulfate | - |
r | |
| formaldehyde + reduced phenazine methosulfate | the enzyme is 100times more efficient at oxidizing formaldehyde than methanol | Methylorubrum extorquens | methanol + phenazine methosulfate | - |
r | |
| methanol + phenazine methosulfate | - |
Methylorubrum extorquens | formaldehyde + reduced phenazine methosulfate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | x * 61000, SDS-PAGE | Methylorubrum extorquens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exaF | - |
Methylorubrum extorquens |
| pyrroloquinoline quinone ethanol dehydrogenase | - |
Methylorubrum extorquens |
| quinoprotein ethanol dehydrogenase | - |
Methylorubrum extorquens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | - |
Methylorubrum extorquens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
methanol | at pH 9.0 and 30°C | Methylorubrum extorquens | |
| 20 | - |
acetaldehyde | at pH 9.0 and 30°C | Methylorubrum extorquens | |
| 259 | - |
formaldehyde | at pH 9.0 and 30°C | Methylorubrum extorquens | |
| 14500 | - |
ethanol | at pH 9.0 and 30°C | Methylorubrum extorquens | |
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Release 2023.1 (January 2023)
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