Literature summary for 1.1.1.B57 extracted from

Vitale, A.; Rosso, F.; Barbarisi, A.; Labella, T.; D'Auria, S.
Properties and evolution of an alcohol dehydrogenase from the Crenarchaeota Pyrobaculum aerophilum (2010), Gene, 461, 26-31.

Search for more literature for 1.1.1.B57

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Pyrobaculum aerophilum

General Stability

General Stability	Organism
Zn2+ is not essential for structural stability of the enzyme	Pyrobaculum aerophilum

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	1 mM, 20% decrease in activity	Pyrobaculum aerophilum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.4	-	3,4-dihydro-1(2H)-naphthalenone	95°C, pH 7.5	Pyrobaculum aerophilum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	important for activity	Pyrobaculum aerophilum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38580	-	x * 38580, calculated from sequence	Pyrobaculum aerophilum
40000	-	x * 40000, SDS-PAGE	Pyrobaculum aerophilum

Organism

Organism	UniProt	Comment	Textmining
Pyrobaculum aerophilum	Q8ZUP0	-	-
Pyrobaculum aerophilum DSM 7523	Q8ZUP0	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrobaculum aerophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3	-	60°C, pH 7.5	Pyrobaculum aerophilum
50	-	95°C, pH 7.5	Pyrobaculum aerophilum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+	3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone. The enzyme does not show activity on a large number of alcohols, aldehydes or ketones. It is active only with alpha-tetralone as substrate	Pyrobaculum aerophilum	1,2,3,4-tetrahydronaphthalen-1-ol + NADP+	-	?
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+	3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone. The enzyme does not show activity on a large number of alcohols, aldehydes or ketones. It is active only with alpha-tetralone as substrate	Pyrobaculum aerophilum DSM 7523	1,2,3,4-tetrahydronaphthalen-1-ol + NADP+	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000, SDS-PAGE	Pyrobaculum aerophilum
?	x * 38580, calculated from sequence	Pyrobaculum aerophilum

Synonyms

Synonyms	Comment	Organism
PAE2687	locus name	Pyrobaculum aerophilum
PyAeADHII	-	Pyrobaculum aerophilum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
95	-	reaction rate increases with temperature up to 95°C	Pyrobaculum aerophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1719	-	3,4-dihydro-1(2H)-naphthalenone	95°C, pH 7.5	Pyrobaculum aerophilum

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Release 2023.1 (January 2023)