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Literature summary for 1.1.1.B52 extracted from

  • Zhang, W.X.; Xu, G.C.; Huang, L.; Pan, J.; Yu, H.L.; Xu, J.H.
    Highly efficient synthesis of (R)-3-quinuclidinol in a space-time yield of 916 g L(-1) d(-1) using a new bacterial reductase ArQR (2013), Org. Lett., 15, 4917-4919.
    View publication on PubMed
Search for more literature for 1.1.1.B52

Application

Application Comment Organism
pharmacology stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals, high yield of (R)-3-quinuclidinol up to 916 g/L * d using a bioreduction approach Agrobacterium tumefaciens
synthesis stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals, high yield of (R)-3-quinuclidinol up to 916 g/L * d using a bioreduction approach Agrobacterium tumefaciens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 3-quinuclidinone pH 7.0, temperature not specified in the publication Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens G1K3P5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-quinuclidinone + NADH + H+ high activity and excellent enantioselectivity Agrobacterium tumefaciens (R)-3-quinuclidinol + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
ArQR
-
 Agrobacterium tumefaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
290
-
 3-quinuclidinone pH 7.0, temperature not specified in the publication Agrobacterium tumefaciens