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Literature summary for 1.1.1.B52 extracted from

  • Isotani, K.; Kurokawa, J.; Suzuki, F.; Nomoto, S.; Negishi, T.; Matsuda, M.; Itoh, N.
    Gene cloning and characterization of two NADH-dependent 3-quinuclidinone reductases from Microbacterium luteolum JCM 9174 (2012), Appl. Environ. Microbiol., 79, 1378-1384.
    View publication on PubMed
Search for more literature for 1.1.1.B52

Application

Application Comment Organism
pharmacology stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals Microbacterium luteolum
synthesis stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals Microbacterium luteolum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. The gene is fused with the His6 tag and thrombin recognition sequence at the N-terminus under the control of the T7 promoter, 3-quinuclidinone reductase BacC Microbacterium luteolum
expression in Escherichia coli. The gene is fused with the His6 tag and thrombin recognition sequence at the N-terminus under the control of the T7 promoter, 3-quinuclidinone reductase QNR Microbacterium luteolum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 NADH pH 7.0, 25°C, 3-quinuclidinone reductase QNR Microbacterium luteolum
0.03
-
 NADH pH 7.0, 25°C, 3-quinuclidinone reductase BacC Microbacterium luteolum
6.5
-
 3-quinuclidinone pH 7.0, 25°C, 3-quinuclidinone reductase QNR Microbacterium luteolum
13.8
-
 3-quinuclidinone pH 7.0, 25°C, 3-quinuclidinone reductase BacC Microbacterium luteolum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30600
-
 3 * 30600, SDS-PAGE, 3-quinuclidinone reductase QNR Microbacterium luteolum
32600
-
 2 * 32600, SDS-PAGE, 3-quinuclidinone reductase BacC Microbacterium luteolum
72000
-
 gel filtration, 3-quinuclidinone reductase BacC Microbacterium luteolum
94000
-
 gel filtration, 3-quinuclidinone reductase QNR Microbacterium luteolum

Organism

Organism UniProt Comment Textmining
Microbacterium luteolum L8B2H6
-
-
Microbacterium luteolum JCM 9174 L8B2H6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Microbacterium luteolum
3-quinuclidinone reductase QNR Microbacterium luteolum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
 pH 7.0, 25°C, 3-quinuclidinone reductase BacC Microbacterium luteolum
8.4
-
 pH 7.0, 25°C Microbacterium luteolum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetylpyridine + NADH + H+ activity is 16.8% compared to the activity with 3-quinuclidinone, 3-quinuclidinone reductase BacC Microbacterium luteolum ?
-
 ?
2-acetylpyridine + NADH + H+ activity is 16.8% compared to the activity with 3-quinuclidinone, 3-quinuclidinone reductase BacC Microbacterium luteolum JCM 9174 ?
-
 ?
3-quinuclidinone + NADH + H+ 3-quinuclidinone (5% w/v, 313 mM) is reduced to (R)-3-quinuclidinol with a molar conversion yield of 100% by 3-quinuclidinone reductase QNR. The optical purity is above 99.9%. No activity with NADPH. The enzyme is strictly specific for 3-quinuclidinone and shows no activity toward several ketones, including tropinone Microbacterium luteolum (R)-3-quinuclidinol + NAD+
-
 ?
3-quinuclidinone + NADH + H+ 3-quinuclidinone (5% w/v, 313 mM) is reduced to (R)-3-quinuclidinol with a molar conversion yield of 94% bei 3-quinuclidinone reductase BacC. The optical purity is above 99.9%. No activity with NADPH Microbacterium luteolum (R)-3-quinuclidinol + NAD+
-
 ?
3-quinuclidinone + NADH + H+ 3-quinuclidinone (5% w/v, 313 mM) is reduced to (R)-3-quinuclidinol with a molar conversion yield of 100% by 3-quinuclidinone reductase QNR. The optical purity is above 99.9%. No activity with NADPH. The enzyme is strictly specific for 3-quinuclidinone and shows no activity toward several ketones, including tropinone Microbacterium luteolum JCM 9174 (R)-3-quinuclidinol + NAD+
-
 ?
3-quinuclidinone + NADH + H+ 3-quinuclidinone (5% w/v, 313 mM) is reduced to (R)-3-quinuclidinol with a molar conversion yield of 94% bei 3-quinuclidinone reductase BacC. The optical purity is above 99.9%. No activity with NADPH Microbacterium luteolum JCM 9174 (R)-3-quinuclidinol + NAD+
-
 ?
7-oxabicyclo[4.1.0]heptan-2-one + NADH + H+ activity is 27.8% compared to the activity with 3-quinuclidinone, 3-quinuclidinone reductase BacC Microbacterium luteolum ?
-
 ?
7-oxabicyclo[4.1.0]heptan-2-one + NADH + H+ activity is 27.8% compared to the activity with 3-quinuclidinone, 3-quinuclidinone reductase BacC Microbacterium luteolum JCM 9174 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32600, SDS-PAGE, 3-quinuclidinone reductase BacC Microbacterium luteolum
trimer 3 * 30600, SDS-PAGE, 3-quinuclidinone reductase QNR Microbacterium luteolum

Synonyms

Synonyms Comment Organism
BacC bacC isolated from Microbacterium luteolum shows 99.9% identity (100% amino acid identity) with the putative oxidoreductase gene bacC of the bacilysin biosynthetic gene cluster derived from Bacillus subtilis, suggesting that bacC is horizontally transferred from Bacillus species Microbacterium luteolum
BacC
-
 Microbacterium luteolum
QNR
-
 Microbacterium luteolum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Microbacterium luteolum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 3-quinuclidinone reductase QNR Microbacterium luteolum
7
-
 3-quinuclidinone reductase BacC Microbacterium luteolum

Cofactor

Cofactor Comment Organism Structure
NADH no activity with NADPH, 3-quinuclidinone reductase QNR Microbacterium luteolum
NADH no activity with NADPH, 3-quinuclidinone reductase BacC Microbacterium luteolum