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Literature summary for 1.1.1.B4 extracted from

  • Zhou, Y.; Peng, Q.; Zhang, L.; Cheng, S.; Zeng, L.; Dong, F.; Yang, Z.
    Characterization of enzymes specifically producing chiral flavor compounds (R)- and (S)-1-phenylethanol from tea (Camellia sinensis) flowers (2019), Food Chem., 280, 27-33 .
    View publication on PubMed
Search for more literature for 1.1.1.B4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells and Arabidopsis thaliana protoplast Camellia sinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Camellia sinensis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the addition of Mg2+ does not affect the enzyme activity significantly Camellia sinensis

Organism

Organism UniProt Comment Textmining
Camellia sinensis A0A494WKI6 cultivar Jinxuan
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose column chromatography Camellia sinensis

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Camellia sinensis
-
leaf
-
 Camellia sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetophenone + NADPH + H+
-
 Camellia sinensis (1R)-1-phenylethanol + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 28660, calculated from amino acid sequence Camellia sinensis

Synonyms

Synonyms Comment Organism
(R)-1-phenylethanolsynthase
-
 Camellia sinensis
RPES
-
 Camellia sinensis
SDR
-
 Camellia sinensis
short chain dehydrogenase
-
 Camellia sinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Camellia sinensis

Cofactor

Cofactor Comment Organism Structure
NADPH strong preference for NADPH as cofactor Camellia sinensis