Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 5 mM, 93% of initial activity | Gluconobacter oxydans | |
Fe3+ | 0.5 mM, 68% of initial activity | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
NADPH | pH 7.4, 30°C | Gluconobacter oxydans | |
0.72 | - |
2,3-Butanedione | pH 7.4, 30°C | Gluconobacter oxydans | |
1.27 | - |
ethyl 4-chloro-3-oxobutanoate | pH 7.4, 30°C | Gluconobacter oxydans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 156% of initial activity | Gluconobacter oxydans | |
Mg2+ | 5 mM, 109% of initial activity | Gluconobacter oxydans | |
NH4+ | 5 mM, 124% of initial activity | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Gluconobacter oxydans DSM 2343 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phenyl-1,2-propandione + NADPH + H+ | - |
Gluconobacter oxydans | ? + NADP+ | - |
? | |
1-phenyl-1,2-propandione + NADPH + H+ | - |
Gluconobacter oxydans DSM 2343 | ? + NADP+ | - |
? | |
2,3-butanedione + NADPH + H+ | - |
Gluconobacter oxydans | (R)-2-hydroxy-3-oxo-butane + NADP+ | - |
? | |
2,3-butanedione + NADPH + H+ | - |
Gluconobacter oxydans DSM 2343 | (R)-2-hydroxy-3-oxo-butane + NADP+ | - |
? | |
2,3-pentanedione + NADPH + H+ | - |
Gluconobacter oxydans | (R)-2-hydroxy-3-oxo-pentane + NADP+ | - |
? | |
4-phenylbutan-2-one + NADPH + H+ | - |
Gluconobacter oxydans | (2R)-4-phenylbutan-2-ol + NADP+ | 99% conversion, 97.9% enantiomeric excess | ? | |
ethyl 2-oxo-4-phenylbutanoate + NADPH + H+ | - |
Gluconobacter oxydans | ethyl (2S)-2-hydroxy-4-phenylbutanoate + NADP+ | 93% conversion, 99% enantiomeric excess | ? | |
ethyl 2-oxo-4-phenylbutanoate + NADPH + H+ | - |
Gluconobacter oxydans DSM 2343 | ethyl (2S)-2-hydroxy-4-phenylbutanoate + NADP+ | 93% conversion, 99% enantiomeric excess | ? | |
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ | - |
Gluconobacter oxydans | ethyl (3S)-4-chloro-3-hydroxybutanoate + NADP+ | 73.4% conversion, 99% enantiomeric excess | ? | |
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ | - |
Gluconobacter oxydans DSM 2343 | ethyl (3S)-4-chloro-3-hydroxybutanoate + NADP+ | 73.4% conversion, 99% enantiomeric excess | ? | |
ethyl oxo(phenyl)acetate + NADPH + H+ | - |
Gluconobacter oxydans | ethyl (2S)-hydroxy(phenyl)acetate + NADP+ | - |
? | |
additional information | the enzyme does not obey Prelog's rule and exhibits anti-Prelog enantiopreference. Hydride transfer occurs at the si faces of carbonyl group for ethyl 4-chloro-3-oxobutanoate (COBE), which is then selectively reduced to the chiral (S)-alcohol. Aromatic ketones are reduced to (R)-enantiomers, whereas keto esters are reduced to (S)-hydroxy esters with different enantioselectivities | Gluconobacter oxydans | ? | - |
? | |
additional information | the enzyme does not obey Prelog's rule and exhibits anti-Prelog enantiopreference. Hydride transfer occurs at the si faces of carbonyl group for ethyl 4-chloro-3-oxobutanoate (COBE), which is then selectively reduced to the chiral (S)-alcohol. Aromatic ketones are reduced to (R)-enantiomers, whereas keto esters are reduced to (S)-hydroxy esters with different enantioselectivities | Gluconobacter oxydans DSM 2343 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Gluconobacter oxydans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
ethyl 4-chloro-3-oxobutanoate | pH 7.4, 30°C | Gluconobacter oxydans | |
36.1 | - |
2,3-Butanedione | pH 7.4, 30°C | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADH | Gluconobacter oxydans | |
NADPH | - |
Gluconobacter oxydans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.1 | - |
ethyl 4-chloro-3-oxobutanoate | pH 7.4, 30°C | Gluconobacter oxydans | |
50.1 | - |
2,3-Butanedione | pH 7.4, 30°C | Gluconobacter oxydans |