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Literature summary for 1.1.1.B3 extracted from

  • Knaus, T.; Cariati, L.; Masman, M.F.; Mutti, F.G.
    In vitro biocatalytic pathway design orthogonal network for the quantitative and stereospecific amination of alcohols (2017), Org. Biomol. Chem., 15, 8313-8325 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.B3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43 cells Thermoanaerobacter ethanolicus

Protein Variants

Protein Variants Comment Organism
I86A the mutant shows reversed stereoselectivity (R) compared to the wild type enzyme Thermoanaerobacter ethanolicus
W110A the mutant shows imperfect wild type stereoselectivity (S) Thermoanaerobacter ethanolicus
W110A/I86A the mutant enzyme is completely non-stereoselective for the reduction of 2-hexanone.Additionally, an extremely low level of stereoselectivity (i.e. from 10% to 24%) is observed for the reduction of aryl-aliphatic ketones and aliphatic ketones Thermoanaerobacter ethanolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Thermoanaerobacter ethanolicus

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter ethanolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin column chromatography Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-1-phenyl 2-propanol + NADP+
-
 Thermoanaerobacter ethanolicus phenylacetone + NADPH + H+
-
 ?
1-(3-fluorophenyl)ethan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(3-fluorophenyl)ethan-1-one + NADPH + H+
-
 ?
1-(3-methoxyphenyl)propan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(3-methoxyphenyl)propan-2-one + NADPH + H+
-
 ?
1-(3-methylphenyl)ethan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(3-methylphenyl)ethan-1-one + NADPH + H+
-
 ?
1-(4-fluorophenyl)ethan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(4-fluorophenyl)ethan-1-one + NADPH + H+
-
 ?
1-(4-fluorophenyl)propan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(4-fluorophenyl)propan-2-one + NADPH + H+
-
 ?
1-(4-methylphenyl)ethan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(4-methylphenyl)ethan-1-one + NADPH + H+
-
 ?
1-(4-methylphenyl)propan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(4-methylphenyl)propan-2-one + NADPH + H+
-
 ?
1-phenoxypropan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-phenoxypropan-2-one + NADPH + H+
-
 ?
1-phenylbutan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-phenylbutan-2-one + NADPH + H+
-
 ?
1-phenylpropan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-phenylpropan-2-one + NADPH + H+
-
 ?
4-methylpentan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 4-methylpentan-2-one + NADPH + H+
-
 ?
4-phenylbutan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 4-phenylbutan-2-one + NADPH + H+
-
 ?
heptan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus heptanal + NADPH + H+
-
 ?
heptan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus heptan-2-one + NADPH + H+
-
 ?
hexan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus hexanal + NADPH + H+
-
 ?
hexan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus hexan-2-one + NADPH + H+
-
 ?
octan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus octanal + NADPH + H+
-
 ?
octan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus octan-2-one + NADPH + H+
-
 ?
pentan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus pentanal + NADPH + H+
-
 ?
pentan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus pentan-2-one + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
S-ADH
-
 Thermoanaerobacter ethanolicus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Thermoanaerobacter ethanolicus