Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 2 mM, no% residual activity | Nocardia fusca AKU 2123 | |
CdCl2 | 2 mM, 6.6% residual activity | Nocardia fusca AKU 2123 | |
EDTA | 2 mM, 56% residual activity | Nocardia fusca AKU 2123 | |
HgCl2 | 2 mM, no residual activity | Nocardia fusca AKU 2123 | |
iodoacetate | 2 mM, 67% residual activity | Nocardia fusca AKU 2123 | |
o-phenanthroline | 2 mM, 0.4% residual activity | Nocardia fusca AKU 2123 | |
trypaflavine | 1 mM, 7.7% residual activity | Nocardia fusca AKU 2123 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
(S)-2-hexanol | 30°C, pH 7.5 | Nocardia fusca AKU 2123 | |
1.6 | - |
(S)-3-pentyn-2-ol | 30°C, pH 7.5 | Nocardia fusca AKU 2123 | |
2.5 | - |
2-Hexanone | 30°C, pH 7.5 | Nocardia fusca AKU 2123 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
4 * 39000, SDS-PAGE | Nocardia fusca AKU 2123 |
150000 | - |
gel filtration | Nocardia fusca AKU 2123 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia fusca AKU 2123 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Nocardia fusca AKU 2123 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41.5 | - |
30°C, pH 7.5 | Nocardia fusca AKU 2123 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-1-phenylethanol + NAD+ | 0.9% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | 1-phenylethanone + NADH + H+ | - |
r | |
(R)-1-phenylethanol + NAD+ | 21% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | 1-phenylethanone + NADH + H+ | - |
r | |
(R)-2-butanol + NAD+ | 6% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | 2-butanone + NADH + H+ | - |
r | |
(R)-2-hexanol + NAD+ | 30% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | 2-hexanone + NADH + H+ | - |
r | |
(R)-2-pentanol + NAD+ | 15% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | 2-pentanone + NADH + H+ | - |
r | |
(R)-3-butyn-2-ol + NAD+ | 0.5% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | but-3-yn-2-one + NADH + H+ | - |
r | |
(R)-3-pentyn-2-ol + NAD+ | 5.6% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | pent-3-yn-2-one + NADH + H+ | - |
r | |
(S)-2-butanol + NAD+ | 27% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | 2-butanone + NADH + H+ | - |
r | |
(S)-2-hexanol + NAD+ | 121% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | 2-hexanone + NADH + H+ | - |
r | |
(S)-2-hexanol + NAD+ | reaction is stereoselective | Nocardia fusca AKU 2123 | 2-hexanone + NADH + H+ | - |
r | |
(S)-2-pentanol + NAD+ | 91% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | 2-pentanone + NADH + H+ | - |
r | |
(S)-3-butyn-2-ol + NAD+ | 6.9% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | but-3-yn-2-one + NADH + H+ | - |
r | |
(S)-3-pentyn-2-ol + NAD+ | 33% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | pent-3-yn-2-one + NADH + H+ | - |
r | |
1-hexanal + NADH + H+ | 254% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | hexan-1-ol + NAD+ | - |
r | |
2-decanone + NADH + H+ | 159% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | (S)-2-decanol + NAD+ | reaction is stereoselective | r | |
2-heptanone + NADH + H+ | 334% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | (S)-2-heptanol + NAD+ | reaction is stereoselective | r | |
2-hexanone + NADH + H+ | 225% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | (S)-2-hexanol + NAD+ | reaction is stereoselective | r | |
2-octanone + NADH + H+ | 223% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | (S)-2-octanol + NAD+ | reaction is stereoselective | r | |
6-methyl-5-hepten-2-one + NADH + H+ | - |
Nocardia fusca AKU 2123 | (S)-6-methyl-5-hepten-2-ol + NAD+ | 188% of the activity with (RS)-2-hexanol | r | |
ethyl (R)-4-chloro-3-hydroxybutanoate + NAD+ | - |
Nocardia fusca AKU 2123 | ethyl 4-chloro-3-oxobutanoate + NADH + H+ | 38.1% of the activity with (RS)-2-hexanol | r | |
ethyl 4-chloro-3-oxobutanoate + NADH + H+ | - |
Nocardia fusca AKU 2123 | ethyl (R)-4-chloro-3-hydroxybutanoate + NAD+ | - |
r | |
additional information | primary alcohols are not accepted as substrate | Nocardia fusca AKU 2123 | ? | - |
? | |
n-valeraldehyde + NADH + H+ | 84% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | ? + NAD+ | - |
r | |
pyruvic acid ethyl ester + NADH + H+ | 225% of the activity with (RS)-2-hexanol | Nocardia fusca AKU 2123 | ? + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 39000, SDS-PAGE | Nocardia fusca AKU 2123 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
oxidation reaction | Nocardia fusca AKU 2123 |
65 | - |
reduction reaction | Nocardia fusca AKU 2123 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, pH 7.0, 85% residual activity | Nocardia fusca AKU 2123 |
50 | - |
30 min, pH 7.0, 50% residual activity | Nocardia fusca AKU 2123 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | reduction reaction | Nocardia fusca AKU 2123 |
8.5 | 9.5 | oxidation reaction | Nocardia fusca AKU 2123 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | 30 min, 30°C, more than 90% residual activity | Nocardia fusca AKU 2123 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | NADPH cannot substitute for NADH | Nocardia fusca AKU 2123 | |
NAD+ | - |
Nocardia fusca AKU 2123 | |
NADH | - |
Nocardia fusca AKU 2123 |