Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.B3 extracted from

  • Xie, S.-X.; Ogawa, J.; Shimizu, S.
    NAD+-Dependent (S)-specific secondary alcohol dehydrogenase involved in stereoinversion of 3-pentyn-2ol catalyzed by Nocardia fusca AKU 2123 (1999), Biosci. Biotechnol. Biochem., 63, 1721-1729.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 2 mM, no% residual activity Nocardia fusca AKU 2123
CdCl2 2 mM, 6.6% residual activity Nocardia fusca AKU 2123
EDTA 2 mM, 56% residual activity Nocardia fusca AKU 2123
HgCl2 2 mM, no residual activity Nocardia fusca AKU 2123
iodoacetate 2 mM, 67% residual activity Nocardia fusca AKU 2123
o-phenanthroline 2 mM, 0.4% residual activity Nocardia fusca AKU 2123
trypaflavine 1 mM, 7.7% residual activity Nocardia fusca AKU 2123

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
(S)-2-hexanol 30°C, pH 7.5 Nocardia fusca AKU 2123
1.6
-
(S)-3-pentyn-2-ol 30°C, pH 7.5 Nocardia fusca AKU 2123
2.5
-
2-Hexanone 30°C, pH 7.5 Nocardia fusca AKU 2123

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
4 * 39000, SDS-PAGE Nocardia fusca AKU 2123
150000
-
gel filtration Nocardia fusca AKU 2123

Organism

Organism UniProt Comment Textmining
Nocardia fusca AKU 2123
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nocardia fusca AKU 2123

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
41.5
-
30°C, pH 7.5 Nocardia fusca AKU 2123

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1-phenylethanol + NAD+ 0.9% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 1-phenylethanone + NADH + H+
-
r
(R)-1-phenylethanol + NAD+ 21% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 1-phenylethanone + NADH + H+
-
r
(R)-2-butanol + NAD+ 6% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 2-butanone + NADH + H+
-
r
(R)-2-hexanol + NAD+ 30% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 2-hexanone + NADH + H+
-
r
(R)-2-pentanol + NAD+ 15% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 2-pentanone + NADH + H+
-
r
(R)-3-butyn-2-ol + NAD+ 0.5% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 but-3-yn-2-one + NADH + H+
-
r
(R)-3-pentyn-2-ol + NAD+ 5.6% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 pent-3-yn-2-one + NADH + H+
-
r
(S)-2-butanol + NAD+ 27% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 2-butanone + NADH + H+
-
r
(S)-2-hexanol + NAD+ 121% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 2-hexanone + NADH + H+
-
r
(S)-2-hexanol + NAD+ reaction is stereoselective Nocardia fusca AKU 2123 2-hexanone + NADH + H+
-
r
(S)-2-pentanol + NAD+ 91% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 2-pentanone + NADH + H+
-
r
(S)-3-butyn-2-ol + NAD+ 6.9% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 but-3-yn-2-one + NADH + H+
-
r
(S)-3-pentyn-2-ol + NAD+ 33% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 pent-3-yn-2-one + NADH + H+
-
r
1-hexanal + NADH + H+ 254% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 hexan-1-ol + NAD+
-
r
2-decanone + NADH + H+ 159% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 (S)-2-decanol + NAD+ reaction is stereoselective r
2-heptanone + NADH + H+ 334% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 (S)-2-heptanol + NAD+ reaction is stereoselective r
2-hexanone + NADH + H+ 225% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 (S)-2-hexanol + NAD+ reaction is stereoselective r
2-octanone + NADH + H+ 223% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 (S)-2-octanol + NAD+ reaction is stereoselective r
6-methyl-5-hepten-2-one + NADH + H+
-
Nocardia fusca AKU 2123 (S)-6-methyl-5-hepten-2-ol + NAD+ 188% of the activity with (RS)-2-hexanol r
ethyl (R)-4-chloro-3-hydroxybutanoate + NAD+
-
Nocardia fusca AKU 2123 ethyl 4-chloro-3-oxobutanoate + NADH + H+ 38.1% of the activity with (RS)-2-hexanol r
ethyl 4-chloro-3-oxobutanoate + NADH + H+
-
Nocardia fusca AKU 2123 ethyl (R)-4-chloro-3-hydroxybutanoate + NAD+
-
r
additional information primary alcohols are not accepted as substrate Nocardia fusca AKU 2123 ?
-
?
n-valeraldehyde + NADH + H+ 84% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 ? + NAD+
-
r
pyruvic acid ethyl ester + NADH + H+ 225% of the activity with (RS)-2-hexanol Nocardia fusca AKU 2123 ? + NAD+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 39000, SDS-PAGE Nocardia fusca AKU 2123

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
oxidation reaction Nocardia fusca AKU 2123
65
-
reduction reaction Nocardia fusca AKU 2123

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
30 min, pH 7.0, 85% residual activity Nocardia fusca AKU 2123
50
-
30 min, pH 7.0, 50% residual activity Nocardia fusca AKU 2123

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6.5 reduction reaction Nocardia fusca AKU 2123
8.5 9.5 oxidation reaction Nocardia fusca AKU 2123

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9.5 30 min, 30°C, more than 90% residual activity Nocardia fusca AKU 2123

Cofactor

Cofactor Comment Organism Structure
additional information NADPH cannot substitute for NADH Nocardia fusca AKU 2123
NAD+
-
Nocardia fusca AKU 2123
NADH
-
Nocardia fusca AKU 2123